Commercial Levels of Chymosin Production by Aspergillus

Autor:	Enrique F. Baliu, Henry G. Heinsohn, Randy M. Berka, Nigel Dunn-Coleman, Ben Bower, Nancy Robinson, Lori J. Wilson, Michael Ward, Katherine H. Kodama, Gina L. Carter, Peggy Bloebaum, Regina LaCost, Elizabeth A. Bodie, Melinda Przetak, Michael H. Lamsa, Gale L. Armstrong
Rok vydání:	1991
Předmět:	chemistry.chemical_classification Aspergillus Neurospora crassa biology Aspergillus niger Mutagenesis (molecular biology technique) Drug Resistance Microbial Fungi imperfecti Deoxyglucose biology.organism_classification Recombinant Proteins chemistry.chemical_compound Enzyme Biosynthesis chemistry Biochemistry Mutagenesis Chymosin Site-directed mutagenesis Biotechnology
Zdroj:	Bio/Technology. 9:976-981
ISSN:	0733-222X
DOI:	10.1038/nbt1091-976
Popis:	We have increased the production of bovine chymosin in Aspergillus niger var. awamori to more than one gram per liter of secreted authentic enzyme by combining a mutagenesis protocol with a novel robotic screening program. Analysis of the superior chymosin producing strains indicated that they have enhanced capabilities to secrete extracellular proteins.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::25913825298ecc5a6a97efb9b3dc1968 https://doi.org/10.1038/nbt1091-976 Zobrazit plný text záznamu Full text from SpringerLink