IL-1 beta Induces a Proinflammatory Fibroblast Microenvironment that Impairs Lung Progenitors' Function
| Autor: | Chiara Ciminieri, Manon E. Woest, Niki L. Reynaert, Irene H. Heijink, René Wardenaar, Diana C. J. Spierings, Corry-Anke Brandsma, Melanie Königshoff, Reinoud Gosens |
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| Přispěvatelé: | Molecular Pharmacology, Groningen Research Institute for Asthma and COPD (GRIAC), Damage and Repair in Cancer Development and Cancer Treatment (DARE), Stem Cell Aging Leukemia and Lymphoma (SALL), Pulmonologie, RS: NUTRIM - R3 - Respiratory & Age-related Health |
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | American Journal of Respiratory Cell and Molecular Biology, 68(4), 444-455. AMER THORACIC SOC American Journal of Respiratory Cell and Molecular Biology, 68(4), 444-455. American Thoracic Society |
| ISSN: | 1044-1549 |
| Popis: | Chronic obstructive pulmonary disease (COPD) is characterized by a persistent inflammatory state in the lungs and defective tissue repair. Although the inflammatory response in patients with COPD is well characterized and known to be exaggerated during exacerbations, its contribution to lung injury and abnormal repair is still unclear. In this study, we aimed to investigate how the inflammatory microenvironment affects the epithelial progenitors and their supporting mesenchymal niche cells involved in tissue repair of the distal lung. We focused on IL-1 beta, a key inflammatory mediator that is increased during exacerbations of COPD, and used an organoid model of lung epithelial cells and fibroblasts to assess the effect of IL-1 beta treatment on these cells' transcriptome and secreted factors. Whereas direct treatment of the lung organoids with IL-1 beta promoted organoid growth, this switched toward inhibition when it was added as fibroblast pretreatment followed by organoid treatment. We then investigated the IL-1 beta-driven mechanisms in the fibroblasts and found an inflammatory response related to (C-X-Cmotif) ligand (CXCL) chemokines; we confirmed that these chemokines were responsible for the impaired organoid growth and found that targeting their C-X-C chemokine receptors 1/2 (CXCR1/2) receptors or the IL-1 beta intracellular signaling reduced the proinflammatory response and restored organoid growth. These data demonstrate that IL-1 beta alters the fibroblasts' state by promoting a distinct inflammatory response, switching their supportive function on epithelial progenitors toward an inhibitory one in an organoid assay. These results imply that chronic inflammation functions as a shift toward inhibition of repair, thereby contributing to chronic inflammatory diseases like COPD. |
| Databáze: | OpenAIRE |
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