Identification and characterization of nonapeptide targeting a human B cell lymphoma, Raji
| Autor: | Seung Hyun Han, Jae Seong Kang, Woon Kyu Lee, Yun-Jaie Choi, Cheol-Heui Yun, Seckho Ha, Jin Huk Choi, Sung Min Ahn |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Phage display
viruses Immunology Peptide Biology Ligands Jurkat cells Peptide Library Cell Line Tumor Biomarkers Tumor medicine Humans Immunology and Allergy Peptide library Peptide sequence B cell Pharmacology chemistry.chemical_classification Burkitt Lymphoma Molecular biology Raji cell medicine.anatomical_structure chemistry Cell culture Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunoglobulin Heavy Chains Oligopeptides |
| Zdroj: | International Immunopharmacology. 8:852-858 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2008.01.037 |
| Popis: | Here, we identified a novel peptide specifically targeting a human B cell lymphoma, Raji, through a conventional phage display method. The amino acid sequence, 'CTLPHLKMC' was obtained with the highest frequency from a nonapeptide-expressing phage library. The phage clone encoding CTLPHLKMC peptide sequence avidly bound to Raji cells compared with control phage clones. Furthermore, flow-cytometric analysis on the biotinylated synthetic CTLPHLKMC peptide demonstrated the high binding affinity to Raji cells in a dose-dependent manner whereas it has binding activity to neither human peripheral blood mononuclear cells including normal B cell derived from healthy donors nor other leukemia cells including THP-1, HL-60, Jurkat and IM-9. MALDI-TOF mass spectrometry following immunoprecipitation assay showed that a potential host receptor for the peptide is a variable region of human immunoglobulin heavy chain which would be a specific phenotypic marker of Raji. In conclusion, these results suggest that the peptide, 'CTLPHLKMC', is a specific ligand to a Raji cell. |
| Databáze: | OpenAIRE |
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