Antikinetochore and antitopoisomerase I antibodies in systemic scleroderma: comparative study using immunoblotted recombinant antigens, immunofluorescence, and double immunodiffusion
Autor: | Kolacinska-Strasz Z, S. Jablonska, Maria Jarzabek-Chorzelska, M. Blaszczyk, T. P. Chorzelski, G. G. Maul |
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Rok vydání: | 1990 |
Předmět: |
Adult
Immunodiffusion Adolescent Centromere Immunoblotting Fluorescent Antibody Technique Dermatology Spindle Apparatus Immunofluorescence Epitope Scleroderma Antibodies Antigen medicine Humans Antigens Scleroderma Systemic biology medicine.diagnostic_test Autoantibody Nuclear Proteins General Medicine Middle Aged Ouchterlony double immunodiffusion medicine.disease Molecular biology Recombinant Proteins DNA Topoisomerases Type I Immunology biology.protein Female Antibody |
Zdroj: | Archives of dermatological research. 282(2) |
ISSN: | 0340-3696 |
Popis: | In 135 patients with systemic scleroderma, we compared three different methods to determine antinuclear autoantibody (ANA) specificity: indirect immunofluorescence, double immunodiffusion, and, employing recombinant antigens, immunoblotting using both marker autoantigens of this disease. A characteristic Scl-70 antibody pattern was found on HEp-2 cells in 83.8% of the patients, double immunodiffusion was positive for the Scl-70 antibodies in 81.9%, and immunoblot with the recombinant topoisomerase I (Topo I) was positive in 71% of the patients. For the centromere autoantibodies we found a high concordance between the anticentromere antibody (ACA) pattern on HEp-2 cells (27 patients positive) and the detection of recombinant kinetochore in immunoblotting (26 patients positive). The three testing techniques gave comparable results, except that the Topo I recombinant antigen used in immunoblotting reacted strongly with fewer than expected of the known Scl-70-positive sera. However, a method using recombinant antigens expressing all epitopes (rather than one of the epitopes of Topo I) will undoubtedly become the method of choice for detecting antibodies in systemic scleroderma. Using the immunoblotting technique with the recombinant antigens we detected in four patients antibodies against both Topo I and kinetochore. More severe symptoms of systemic scleroderma were found in patients who had both antibodies. The combined presence of both marker autoantibodies is therefore not as rare as previously reported and may predict severe disease. |
Databáze: | OpenAIRE |
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