Expression and Characterization of Cryptococcus neoformans Recombinant App1
| Autor: | Asfia Qureshi, Maurizio Del Poeta, Virginia Williams |
|---|---|
| Rok vydání: | 2011 |
| Předmět: |
Circular dichroism
Insecta Veterinary (miscellaneous) Genetic Vectors Molecular Conformation Gene Expression Biology Applied Microbiology and Biotechnology Microbiology Article Cell Line law.invention Plasmid In vivo law Animals Disulfides Polyacrylamide gel electrophoresis Cryptococcus neoformans Circular Dichroism Lentivirus biology.organism_classification Recombinant Proteins In vitro Molecular Weight Spectrometry Fluorescence Biochemistry Cell culture Chromatography Gel Recombinant DNA Electrophoresis Polyacrylamide Gel Carrier Proteins Agronomy and Crop Science Plasmids |
| Zdroj: | Mycopathologia. 173:395-405 |
| ISSN: | 1573-0832 0301-486X |
| DOI: | 10.1007/s11046-011-9486-7 |
| Popis: | We characterized Cryptococcus neoformans recombinant antiphagocytic protein 1 (rApp1) by SDS-PAGE, gel filtration chromatography, circular dichroism, and fluorescence spectroscopy. rApp1 produced by C. neoformans var. grubii contains an odd number of cysteines; therefore, it has the ability to form intermolecular disulfide bridges which can lead to the formation of amyloid fibrils in the absence of β-mercaptoethanol or DTT in vitro. Alternate approaches to over-expression of rApp1 in the Lepidopteran High Five(™) Insect cell line using pIZ/V5-His and in lentivirus were explored and are described. Finally, localization of App1 in vivo was examined in the presence and absence of the capsule. |
| Databáze: | OpenAIRE |
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