Mobilization of MHC class I molecules from late endosomes to the cell surface following activation of CD34-derived human Langerhans cells
| Autor: | Paul J. Lehner, M. A. Scott, J. P. Luzio, Paul A. MacAry, J. I. O. Craig, Mark E. Lindsay |
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| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharides
Antigen presentation CD1 Antigens CD34 chemical and pharmacologic phenomena Endosomes Primaquine Biology Antigen Tapasin MHC class I Humans Drug Interactions Cells Cultured Microscopy Confocal Multidisciplinary Antigen processing Stem Cells Histocompatibility Antigens Class I Histocompatibility Antigens Class II Biological Transport Dendritic Cells Transporter associated with antigen processing Biological Sciences MHC restriction Cell biology Phenotype Langerhans Cells biology.protein |
| Zdroj: | Proceedings of the National Academy of Sciences. 98:3982-3987 |
| ISSN: | 1091-6490 0027-8424 |
| Popis: | Langerhans cells are a subset of dendritic cells (DCs) found in the human epidermis with unique morphological and molecular properties that enable their function as “sentinels” of the immune system. DCs are pivotal in the initiation and regulation of primary MHC class I restricted T lymphocyte immune responses and are able to present both endogenous and exogenous antigen onto class I molecules. Here, we study the MHC class I presentation pathway following activation of immature, CD34-derived human Langerhans cells by lipopolysaccharide (LPS). LPS induces an increase in all components of the MHC class I pathway including the transporter for antigen presentation (TAP), tapasin and ERp57, and the immunoproteasome subunits LMP2 and LMP7. Moreover, in CD34-derived Langerhans cells, the rapid increase in expression of MHC class I molecules seen at the cell surface following LPS activation is because of mobilization of MHC class I molecules from HLA-DM positive endosomal compartments, a pathway not seen in monocyte-derived DCs. Mobilization of class I from this compartment is primaquine sensitive and brefeldin A insensitive. These data demonstrate the regulation of the class I pathway in concert with the maturation of the CD34-derived Langerhans cells and suggest potential sites for antigen loading of class I proteins. |
| Databáze: | OpenAIRE |
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