Antimicrobial activity of topical dyes used in clinical veterinary ophthalmology
| Autor: | Fabiano Montiani-Ferreira, Lucianne Leigue dos Santos, Thiago Alegre Coelho Ferreira, Bret A. Moore, José Francisco Ghignatti Warth |
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| Rok vydání: | 2018 |
| Předmět: |
Preservative
Staphylococcus pseudintermedius 040301 veterinary sciences Microbial Sensitivity Tests Bacterial growth Gram-Positive Bacteria Eye Infections Bacterial Microbiology 0403 veterinary science 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lissamine Green Dyes Gram-Negative Bacteria Rose bengal Animals Agar diffusion test Fluorescein Fluorescent Dyes Rose Bengal General Veterinary biology 04 agricultural and veterinary sciences biology.organism_classification Antimicrobial eye diseases chemistry 030221 ophthalmology & optometry Ophthalmic Solutions Antibacterial activity |
| Zdroj: | Veterinary ophthalmologyREFERENCES. 23(3) |
| ISSN: | 1463-5224 |
| Popis: | Objective To evaluate in vitro the antibacterial effects of fluorescein, rose bengal, and lissamine green topical ophthalmic dyes against selected Gram-positive and Gram-negative bacteria, and to evaluate whether preserved or preservative-free fluorescein solutions are able to inhibit or potentiate bacterial growth. Procedures Susceptibility testing was performed using the Kirby-Bauer disk diffusion method plated with clinical ocular isolates of Staphylococcus aureus, Staphylococcus pseudintermedius, Streptococcus spp., Escherichia coli, and Pseudomonas aeruginosa. Bacterial growth inhibition was evaluated 24 hours following the addition of commercially available fluorescein, rose bengal, and lissamine green sterile strips. Antimicrobial effectiveness testing was performed by inoculation of compounded 1% dye solutions, both with and without preservatives (fluorescein and lissamine contained thiomersal, and rose bengal contained nipagin and nepazol), with the five previously mentioned bacteria. Growth was evaluated at days 7, 14, and 28. Results All dyes showed antibacterial activity against Gram-positive organisms. Preservative-free compounded 1% fluorescein solution inhibited growth of Gram-positive organisms but not of Gram-negative organisms. Preservative-free rose bengal and lissamine green inhibited growth of both types of organisms. Conclusions Preferably, ocular surface samples for antimicrobial culture should be taken prior to the administration of topical dyes, due to their potential antibacterial activity, particularly if undiluted strips are applied directly or commercial fluorescein solutions are used and not immediately rinsed. Ophthalmic dye solutions containing preservative are safe from bacterial growth for up to 28 days if properly handled and stored. The use of preservative-free fluorescein solutions should be avoided and preservative-free rose bengal and lissamine green should be handled carefully. |
| Databáze: | OpenAIRE |
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