Assesment of artificial caries lesions through scanning electron microscopy and cross-sectional microhardness test
| Autor: | Ricardo de Sousa Vieira, Mirian de Waele Souchois de Marsillac |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Adult
Molar Time Factors Materials science Scanning electron microscope Acrylic Resins Dentistry Indentation hardness electron scanning Fluorides Dental Enamel Solubility Hardness Microscopy Humans dental enamel Lactic Acid Tooth Demineralization General Dentistry Anatomy Cross-Sectional business.industry Dental enamel Temperature General Medicine Hydrogen-Ion Concentration ultrastructure lcsh:RK1-715 stomatognathic diseases Durapatite Rough surface lcsh:Dentistry Microscopy Electron Scanning Dental caries microscopy Prism business |
| Zdroj: | Indian Journal of Dental Research, Vol 24, Iss 2, Pp 249-254 (2013) |
| ISSN: | 1998-3603 0970-9290 |
| Popis: | Aims: To assess through scanning electron microscopy (SEM) and cross-sectional microhardness (CSMH) test whether the methodology exposed in this experiment can be used to produce artificial active white spot lesions (AAWSLs) on smooth unabraded human dental enamel. Materials and Methods: Ten human permanent molars were used in this experiment. One section of each tooth was double coated with nail varnish except for a limited central area sized 2.5 mm × 1 mm (2.5 mm 2 ). Each specimen was individually exposed to 10.4 ml of a demineralizing solution at pH 5.0, during 42 days (37°C) without agitation. Samples were sectioned in the center of the AAWSL and one half was analyzed in SEM and the other half was subjected to CSMH. Descriptive statistics was performed to determine mean depth of the lesion. Results: The mean depth of AAWSL was 100 μ m (s.d. =12.1) and a white dull rough surface could be detected by the unaided eye. SEM images demonstrated that although some surface areas of the lesion appeared to be relatively intact, erosion was present. A prismatic pattern of dissolution was observed in all samples with an enlargement of the prism sheaths and some samples had also sites of destruction of prism cores. Conclusion: This methodology can be used to induce AAWSLs in human dental enamel but surface erosion has to be taken into account when performing CSMH test. |
| Databáze: | OpenAIRE |
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