Fatty alcohols production by oleaginous yeast
| Autor: | José L. Adrio, Carmen Ronchel, Armando Lara, Sandy Fillet, Beatriz Suárez, Jordi Gibert |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Sucrose
Fatty acyl-CoA reductase Rhodosporidium toruloides Bioengineering Biology Reductase Applied Microbiology and Biotechnology Metabolic engineering chemistry.chemical_compound Bioreactors Bioreactor Fatty alcohols Basidiomycota Substrate (chemistry) food and beverages Bioenergy/Biofuels/Biochemicals biology.organism_classification equipment and supplies Aldehyde Oxidoreductases Yeast Culture Media chemistry Biochemistry Batch Cell Culture Techniques Fermentation Gammaproteobacteria Biotechnology |
| Zdroj: | Journal of Industrial Microbiology & Biotechnology |
| ISSN: | 1367-5435 |
| DOI: | 10.1007/s10295-015-1674-x |
| Popis: | We have engineered Rhodosporidium toruloides to produce fatty alcohols by expressing a fatty acyl-CoA reductase from Marinobacter aquaeolei VT8. Production of fatty alcohols in flasks was achieved in different fermentation media at titers ranging from 0.2 to 2 g/L. In many of the conditions tested, more than 80 % of fatty alcohols were secreted into the cultivation broth. Through fed-batch fermentation in 7 L bioreactors, over 8 g/L of C16–C18 fatty alcohols were produced using sucrose as the substrate. This is the highest titer ever reported on microbial production of fatty alcohols to date. Electronic supplementary material The online version of this article (doi:10.1007/s10295-015-1674-x) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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