In vitro RNA iodination with the aid of chloramine T
| Autor: | Sergei D. Ivanov, Yuri F. Bobrov, Jacob D. Shaposhnikov, Yuri P. Zerov, Edward A. Ratovitski |
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| Rok vydání: | 1976 |
| Předmět: |
Chloramine
Chemistry Chloramines Biophysics RNA Halogenation Cell Biology Biochemistry Iodine Radioisotopes Combinatorial chemistry In vitro Rats chemistry.chemical_compound Liver High specific activity Isotope Labeling Chloramine-T Centrifugation Density Gradient Animals Electrophoresis Polyacrylamide Gel Molecular Biology |
| Zdroj: | Analytical Biochemistry. 75:234-240 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(76)90074-9 |
| Popis: | A new method for in vitro RNA radioiodination with the aid of chloramine T has been developed. The 125 I-labeled RNA preparations obtained by this method were uniformly labeled, nondegraded, and of high specific activity. The method is simple and the results are reproduced easily. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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