Leishmania major Friedlin chromosome 1 has an unusual distribution of protein-coding genes

Autor:	Patrick Bastien, Peter J. Myler, Theo deVos, Lindsey Audleman, Craig Lemley, Steven Swartzell, Erika Rickel, Alasdair Ivens, Patti Kiser, Greg Hixson, Charles Magness, Guoliang Fu, Thomas Westlake, Ellen Sisk, Kenneth Stuart, Susan M. Sunkin
Rok vydání:	1999
Předmět:	Whole genome sequencing Genetics Multidisciplinary Molecular Sequence Data Protozoan Proteins Nucleic acid sequence Sequence Analysis DNA Genome project Biological Sciences Biology Genome Cot analysis Gene density Animals Humans Repeated sequence Genome Protozoan Gene Leishmania major
Zdroj:	Proceedings of the National Academy of Sciences. 96:2902-2906
ISSN:	1091-6490 0027-8424
DOI:	10.1073/pnas.96.6.2902
Popis:	Leishmania are evolutionarily ancient protozoans (Kinetoplastidae) and important human pathogens that cause a spectrum of diseases ranging from the asymptomatic to the lethal. The Leishmania genome is relatively small [≈34 megabases (Mb)], lacks substantial repetitive DNA, and is distributed among 36 chromosomes pairs ranging in size from 0.3 Mb to 2.5 Mb, making it a useful candidate for complete genome sequence determination. We report here the nucleotide sequence of the smallest chromosome, chr1. The sequence of chr1 has a 257-kilobase region that is densely packed with 79 protein-coding genes. This region is flanked by telomeric and subtelomeric repetitive elements that vary in number and content among the chr1 homologs, resulting in an ≈27.5-kilobase size difference. Strikingly, the first 29 genes are all encoded on one DNA strand, whereas the remaining 50 genes are encoded on the opposite strand. Based on the gene density of chr1, we predict a total of ≈9,800 genes in Leishmania , of which 40% may encode unknown proteins.
Databáze:	OpenAIRE
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