L. plantarum WCFS1 enhances Treg frequencies by activating DCs even in absence of sampling of bacteria in the Peyer Patches
| Autor: | Bruno Pot, Catherine Daniel, Paul de Vos, Miriam Bermudez-Brito, Theo Borghuis, Bart J. de Haan, Marijke M. Faas |
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| Přispěvatelé: | Top Institute Food and Nutrition (TIFN), University Medical Center Groningen [Groningen] (UMCG), University of Groningen [Groningen], Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Centre National de la Recherche Scientifique (CNRS)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Vrije Universiteit Brussel (VUB), This work was supported by a project from the Top Institute Food and Nutrition, Wageningen, The Netherlands., Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Daniel, Catherine, Reproductive Origins of Adult Health and Disease (ROAHD), Translational Immunology Groningen (TRIGR), Man, Biomaterials and Microbes (MBM), Department of Bio-engineering Sciences |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine lcsh:Medicine Immunomodulation/immunology T-Lymphocytes Regulatory INTESTINAL EPITHELIUM law.invention Peyer's Patches Probiotic LACTOBACILLUS-PLANTARUM law lcsh:Science Probiotics/pharmacology INFLUENZA-VIRUS INFECTION Mice Inbred BALB C Multidisciplinary biology IMMUNE-RESPONSES food and beverages T-Lymphocytes Helper-Inducer T helper cell Intestinal epithelium Intestines medicine.anatomical_structure Cytokines [SDV.IMM.IMM] Life Sciences [q-bio]/Immunology/Immunotherapy Spleen/immunology 030106 microbiology chemical and pharmacologic phenomena Spleen T-Lymphocytes Regulatory/immunology CASEI Article CD103(+) DENDRITIC CELLS Immunomodulation 03 medical and health sciences Cytokines/immunology Immune system T-Lymphocytes Helper-Inducer/immunology Journal Article medicine Animals HEALTHY LACTOCOCCUS-LACTIS Probiotics lcsh:R Dendritic Cells [SDV.IMM.IMM]Life Sciences [q-bio]/Immunology/Immunotherapy Intestines/microbiology biology.organism_classification Peyer Patch MICE [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition 030104 developmental biology Dendritic Cells/immunology Immunology Lactobacillus plantarum/immunology Peyer's Patches/immunology bacteria lcsh:Q [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition Lactobacillus plantarum Ex vivo TRACT |
| Zdroj: | Scientific Reports Scientific Reports, Nature Publishing Group, 2018, 8 (1), pp.1785. ⟨10.1038/s41598-018-20243-1⟩ Scientific Reports, Vol 8, Iss 1, Pp 1-10 (2018) Scientific Reports, 2018, 8 (1), pp.1785. ⟨10.1038/s41598-018-20243-1⟩ Scientific Reports, 8(1):1785. Nature Publishing Group |
| ISSN: | 2045-2322 |
| Popis: | Probiotics such as L. plantarum WCFS1 can modulate immune responses in healthy subjects but how this occurs is still largely unknown. Immune-sampling in the Peyer Patches has been suggested to be one of the mechanisms. Here we studied the systemic and intestinal immune effects in combination with a trafficking study through the intestine of a well-established immunomodulating probiotic, i.e. L. plantarum WCFS1. We demonstrate that not more than 2–3 bacteria were sampled and in many animals not any bacterium could be found in the PP. Despite this, L. plantarum was associated with a strong increase in infiltration of regulatory CD103+ DCs and generation of regulatory T cells in the spleen. Also, a reduced splenic T helper cell cytokine response was observed after ex vivo restimulation. L. plantarum enhanced Treg cells and attenuated the T helper 2 response in healthy mice. We demonstrate that, in healthy mice, immune sampling is a rare phenomenon and not required for immunomodulation. Also in absence of any sampling immune activation was found illustrating that host-microbe interaction on the Peyer Patches was enough to induce immunomodulation of DCs and T-cells. |
| Databáze: | OpenAIRE |
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