Cytoskeletal control of alanine transport across the rat and turtle small intestine
| Autor: | Mary E Haddad, Abdo Jurjus, Elias Sarru, Camille F. Nassar |
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| Rok vydání: | 1984 |
| Předmět: |
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Biological Transport Active Biology chemistry.chemical_compound Procaine Intestine Small medicine Colchicine Animals Cytoskeleton Alanine Alanine transport Rats Inbred Strains General Medicine Membrane transport Molecular biology Small intestine Rats Turtles Microscopy Electron medicine.anatomical_structure Biochemistry chemistry Vincristine Intracellular medicine.drug |
| Zdroj: | Comparative biochemistry and physiology. A, Comparative physiology. 79(1) |
| ISSN: | 0300-9629 |
| Popis: | Procaine inhibited significantly (P less than 0.01) alanine accumulation in the rat intestinal strips in a concentration-dependent pattern, whereas it showed no effect on alanine uptake by the turtle intestinal cells. Colchicine and Vinca alkaloids at 5 X 10(-4) and 1.5 X 10(-6) M respectively caused a significant inhibition (P less than 0.01) of intracellular alanine concentration in the rat with no effect noticed in the turtle. Unidirectional influx of alanine across the brush border membrane of the rat was significantly (P less than 0.01) reduced in the presence of procaine, colchicine and vincristine in the preincubation medium. The same drugs did not show any effect on alanine influx into the turtle small intestine. Electron microscopy showed major structural alterations in the cytoskeletal organization of the turtle intestine in response to procaine, colchicine or vincristine treatment. It is proposed that microtubular system may participate in the overall transport mechanism of alanine across the small intestine. |
| Databáze: | OpenAIRE |
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