Functional characterization and fitness cost of spinosad-resistant alleles in Ceratitis capitata

Autor: Vicente Navarro-Llopis, Trent Perry, Lucas Sánchez, Enric Urena, Elena López-Errasquín, Ana Guillem-Amat, Philip J. Batterham, Félix Ortego, Pedro Hernández-Crespo
Přispěvatelé: Comisión Interministerial de Ciencia y Tecnología, CICYT (España), Ministerio de Economía y Competitividad (España), Guillem-Amat, Ana [0000-0002-9775-5260], Ureña, Enric [0000-0003-4873-4735], López-Errasquín, Elena [0000-0003-0104-805X], Navarro-Llopis, Vicente [0000-0003-3030-3304], Batterham, Philip [0000-0001-9840-9119], Sánchez, Lucas [0000-0002-7725-8961], Trent, Perry [0000-0002-8045-0487], Hernández-Crespo, Pedro [0000-0001-9350-3640], Ortego, Félix [0000-0002-7778-8508], Guillem-Amat, Ana, Ureña, Enric, López-Errasquín, Elena, Navarro-Llopis, Vicente, Batterham, Philip, Sánchez, Lucas, Trent, Perry, Hernández-Crespo, Pedro, Ortego, Félix
Rok vydání: 2020
Předmět:
0106 biological sciences
Fitness traits
media_common.quotation_subject
Spinosad
nAChR
medicine.disease_cause
15.- Proteger
restaurar y promover la utilización sostenible de los ecosistemas terrestres
gestionar de manera sostenible los bosques
combatir la desertificación y detener y revertir la degradación de la tierra
y frenar la pérdida de diversidad biológica
01 natural sciences
Competition (biology)
03 medical and health sciences
medicine
Behaviour
Allele
030304 developmental biology
media_common
Genetics
Behavior
0303 health sciences
Mutation
Diamondback moth
biology
NAChR
Medfly
Ceratitis capitata
biology.organism_classification
08.- Fomentar el crecimiento económico sostenido
inclusivo y sostenible
el empleo pleno y productivo
y el trabajo decente para todos
010602 entomology
Capitata
Drosophila melanogaster
GAL4 > UAS
Agronomy and Crop Science
medicine.drug
Zdroj: Digital.CSIC. Repositorio Institucional del CSIC
instname
RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
ISSN: 1612-4766
1612-4758
DOI: 10.1007/s10340-020-01205-x
Popis: 16 p.-6 fig.-2 tab.
The sustainability of control programs for the Mediterranean fruit fly, Ceratitis capitata, for citrus crops in Spain has been threatened by the development of resistance to malathion and lambda-cyhalothrin in recent years. Spinosad is widely used without apparent loss of efficacy. However, a highly resistant strain, JW-100s, has been obtained after laboratory selection. Spinosad resistance in JW-100s has been associated with different mutant alleles of the α6 subunit of the nicotinic acetylcholine receptor (Ccα6) including an isoform-specific truncation allele, Ccα63aQ68*. Using the GAL4 > UAS system in Drosophila melanogaster to demonstrate expression of this truncated α6 subunit, in a dα6 loss-of-function genetic background, does not rescue susceptibility to spinosad, while the expression of Ccα6 wild-type isoforms does. We have also generated C. capitata isolines from JW-100s homozygous for: (1) the Ccα63aQ68*Δ3b-4 allele, which contains the mutation 3aQ68*, and (2) the Ccα63aQ68*–K352* allele, which contains the mutations 3aQ68* and K352*. Neither of these produce complete Ccα6 transcripts. The frequency of resistant alleles declined when in competition with individuals carrying the wild-type allele. Through extensive testing of both biological and behavioral fitness traits, we identified a reduced ability of Ccα63aQ68*Δ3b-4 males to detect the parapheromone and to mate with females carrying the Ccα63aQ68*–K352* allele in competition experiments. Thus, not only the potential for spontaneous resistant mutations to arise in Ccα6 but also their fitness costs must be considered when planning resistance management strategies for C. capitata.
This work received financial support from CICYT(AGL2016-76516-R). The Spanish MINECO granted A. Guillem-Amat a predoc (BES-C-2014-068937) and a mobility (EEBB-I-16-11336) fellowships.
Databáze: OpenAIRE
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