Collagen fibers mediate MRI-detected water diffusion and anisotropy in breast cancers
| Autor: | Dieter Leibfritz, Alireza Akhbardeh, Kristine Glunde, Venu Raman, Michael A. Jacobs, Zaver M. Bhujwalla, Samata Kakkad, Balaji Krishnamachary, Jiangyang Zhang, Desmond Jacob, Meiyappan Solaiyappan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Cancer Research
Pathology HRE hypoxia response element ER estrogen receptor aDW average diffusion weighted FOV field of view 030218 nuclear medicine & medical imaging Mice 0302 clinical medicine ADC apparent diffusion coefficient 2D two-dimensional HER-2 human epidermal growth factor receptor 2 Fiber FA fractional anisotropy RFP red fluorescence protein Chemistry VEGF-A vascular endothelial growth factor A lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ECM extracellular matrix 030220 oncology & carcinogenesis Heterografts IDC invasive ductal carcinoma Female medicine.medical_specialty Original article SHG second harmonic generation Breast Neoplasms lcsh:RC254-282 Collagen Type I 03 medical and health sciences In vivo Cell Line Tumor Fractional anisotropy medicine Effective diffusion coefficient Animals Humans HIF-1 hypoxia inducible factor SCID severe combined immunodeficient Col1 collagen 1 3D three-dimensional Cancer PR progesterone receptor Fibroblasts medicine.disease Collagen type I alpha 1 Disease Models Animal Diffusion Magnetic Resonance Imaging H&E hematoxylin and eosin LN lymph node Anisotropy DTI diffusion tensor imaging PFA paraformaldehyde MRI magnetic resonance imaging Ex vivo Diffusion MRI |
| Zdroj: | Neoplasia (New York, N.Y.) Neoplasia: An International Journal for Oncology Research, Vol 18, Iss 10, Pp 585-593 (2016) |
| ISSN: | 1476-5586 1522-8002 |
| Popis: | Collagen 1 (Col1) fibers play an important role in tumor interstitial macromolecular transport and cancer cell dissemination. Our goal was to understand the influence of Col1 fibers on water diffusion, and to examine the potential of using noninvasive diffusion tensor imaging (DTI) to indirectly detect Col1 fibers in breast lesions. We previously observed, in human MDA-MB-231 breast cancer xenografts engineered to fluoresce under hypoxia, relatively low amounts of Col1 fibers in fluorescent hypoxic regions. These xenograft tumors together with human breast cancer samples were used here to investigate the relationship between Col1 fibers, water diffusion and anisotropy, and hypoxia. Hypoxic low Col1 fiber containing regions showed decreased apparent diffusion coefficient (ADC) and fractional anisotropy (FA) compared to normoxic high Col1 fiber containing regions. Necrotic high Col1 fiber containing regions showed increased ADC with decreased FA values compared to normoxic viable high Col1 fiber regions that had increased ADC with increased FA values. A good agreement of ADC and FA patterns was observed between in vivo and ex vivo images. In human breast cancer specimens, ADC and FA decreased in low Col1 containing regions. Our data suggest that a decrease in ADC and FA values observed within a lesion could predict hypoxia, and a pattern of high ADC with low FA values could predict necrosis. Collectively the data identify the role of Col1 fibers in directed water movement and support expanding the evaluation of DTI parameters as surrogates for Col1 fiber patterns associated with specific tumor microenvironments as companion diagnostics and for staging. |
| Databáze: | OpenAIRE |
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