Conservation of function of Drosophila melanogaster abl and murine v-abl proteins in transformation of mammalian cells
| Autor: | Gina D. Holland, M J Henkemeyer, R Risser, F M Hoffmann, D A Kaehler |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Abelson murine leukemia virus
Immunology Molecular Sequence Data Restriction Mapping Bone Marrow Cells Transfection Microbiology Mice Viral Proteins Virology Drosophilidae hemic and lymphatic diseases Sequence Homology Nucleic Acid Murine leukemia virus medicine Animals Amino Acid Sequence neoplasms Cells Cultured Mice Inbred BALB C ABL biology Chimera Protein-Tyrosine Kinases biology.organism_classification medicine.disease Molecular biology Fusion protein Leukemia Virus Murine Leukemia Cell Transformation Neoplastic Drosophila melanogaster Insect Science DNA Viral DNA Probes Research Article |
| Zdroj: | Journal of virology. 64(5) |
| ISSN: | 0022-538X |
| Popis: | The Drosophila melanogaster abl and the murine v-abl genes encode tyrosine protein kinases (TPKs) whose amino acid sequences are highly conserved. To assess functional conservation between the two gene products, we constructed Drosophila abl/v-abl-chimeric Abelson murine leukemia viruses. In these chimeric Abelson murine leukemia viruses, the TPK and carboxy-terminal regions of v-abl were replaced with the corresponding regions of D. melanogaster abl. The chimeric Abelson murine leukemia viruses were able to mediate morphological and oncogenic transformation of NIH 3T3 cells and were able to abrogate the interleukin-3 dependence of a lymphoid cell line. We also found that a virus that contained both TPK and carboxy-terminal Drosophila abl regions had no in vitro transforming activity for primary bone marrow cells and lacked the ability to induce tumors in susceptible mice. A virus that replaced only a portion of the v-abl TPK region with that of Drosophila abl had low activity in in vitro bone marrow transformation and tumorigenesis assays. These results indicate that the transforming functions of abl TPKs are only partially conserved through evolution. These results also imply that the TPK region of v-abl is a major determinant of its efficient lymphoid cell-transforming activity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|