HGF/NK4 is a specific antagonist for pleiotrophic actions of hepatocyte growth factor
| Autor: | Masao Tanaka, Hideo Shimura, Toshikazu Nakamura, Kunio Matsumoto, Kazuhiko Date |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Liver cytology
Cell Biophysics Biology Kidney Binding Competitive Biochemistry Kringle domain Structure-Activity Relationship chemistry.chemical_compound Dogs Kringles Cell Movement Structural Biology Genetics medicine Animals Humans Phosphorylation Receptor Molecular Biology Cells Cultured Cell Size Hepatocyte growth factor DNA synthesis Tyrosine phosphorylation DNA Cell Biology Proto-Oncogene Proteins c-met Molecular biology HGF/NK4 Peptide Fragments Recombinant Proteins HGF-antagonist Rats medicine.anatomical_structure Liver chemistry Tyrosine Sequence Analysis medicine.drug |
| Zdroj: | FEBS Letters. (1):1-6 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(97)01475-0 |
| Popis: | We prepared a specific antagonist for hepatocyte growth factor (HGF) and designated it HGF/NK4. HGF/NK4 is composed of N-terminal 447 amino acids of the α-chain of HGF, thus contains the N-terminal hairpin domain and subsequent four kringle domains. HGF/NK4 competitively inhibited the specific binding of HGF to the receptor. Importantly, HGF/NK4 neither stimulated DNA synthesis of primary cultured rat hepatocytes (mitogenesis) nor induced cell scattering (motogenesis) and branching tubulogenesis (morphogenesis) of MDCK renal epithelial cells, however, HGF/NK4 almost completely inhibited the mitogenic, motogenic, and morphogenic activities of HGF. HGF/NK4 also suppressed tyrosine phosphorylation of the c-Met/HGF receptor induced by HGF. Apparently this is the first documentation of a specific antagonist which abrogates the mitogenic, motogenic, and morphogenic activities of HGF. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|