Neutrophil chemotaxis on silicone and polyurethane surfaces
| Autor: | Tim Poate, Amy S. Indorf, Robert J. Sherertz |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Neutrophils
Surface Properties Polyurethanes technology industry and agriculture Silicones Zymosan Chemotaxis N-Formylmethionine leucyl-phenylalanine Complement system Catheterization N-Formylmethionine Leucyl-Phenylalanine Catheter chemistry.chemical_compound Chemotaxis Leukocyte Infectious Diseases Silicone Blood chemistry In vivo Immunology Biophysics Immunology and Allergy Agarose Humans Leukocyte chemotaxis |
| Zdroj: | The Journal of infectious diseases. 180(5) |
| ISSN: | 0022-1899 |
| Popis: | Silicone vascular catheters have a greater risk of infection and produce greater inflammation in vivo and greater complement activation in vitro than other vascular catheter polymer materials. This study investigated whether polymorphonuclear leukocyte (PMNL) chemotaxis under agarose on silicone surfaces is different than on polyurethane (PU). Glass slides were coated with silicone and PU by use of a constant-speed dipping apparatus. Chemotaxis (3 h) in response to (10 27 mL) FMLP, zymosan-activated serum, and fresh serum (100%) was greater on silicone than on PU ( ). Polyclonal antibody to C5a blocked 150% of the movement P ! .05 toward serum ( ). Serum in the PMNL well significantly decreased chemotaxis toward P ! .05 FMLP on silicone ( ) but not on PU. These findings suggest that excessive complement P ! .05 activation by silicone may interfere with chemotaxis, but further work is necessary to determine whether this is relevant to an increased risk of catheter-related infection. In a prior study, we demonstrated that silicone catheters had a greater risk of infection than catheters made of other materials (polyurethane [PU], polyvinylchloride, or Teflon) [1]. The mechanism of this effect was not explained, but a possible clue was provided by the paradoxical observation that uninfected silicone catheters invoked a greater inflammatory response than other materials. Greater inflammation around silicone catheters suggested either that the silicone caused more tissue damage, resulting in a greater inflammatory response, or that it generated a stronger chemotactic gradient than other polymer materials. Subsequent in vitro demonstration that silicone catheters produced 10-fold greater complement activation [2] suggested that the effect of silicone on chemotaxis was worth pursuing further. The purpose of this study was to determine whether neutrophil chemotaxis on silicone surfaces is different than on other polymer surfaces. Such data might provide direct evidence of differences in neutrophil function on silicone surfaces in comparison with other polymers. Leukocyte chemotaxis has been studied over the years by a variety of methods, including the Boyden membrane filter method and modifications thereof [3, 4] and a method that uses agarose on polystyrene surfaces [5‐8]. We developed a modified chemotaxis under agarose method that allows the evaluation of neutrophil chemotaxis on polymer-coated slides. |
| Databáze: | OpenAIRE |
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