CDK11(p58) is required for centriole duplication and Plk4 recruitment to mitotic centrosomes
| Autor: | Nathalie Franck, Pierre Romé, Régis Giet, Emilie Montembault, Aude Pascal, Jean-Yves Cremet |
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| Přispěvatelé: | Institut de Génétique et Développement de Rennes (IGDR), Université de Rennes (UR)-Centre National de la Recherche Scientifique (CNRS), RG and NF are funded by the Agence Nationale de la Recherche (programme Jeune Chercheur) and the Ligue Nationale Contre le Cancer (Equipe Labellise). EM and PR are doctoral fellows of the French Ministry of Research., Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), De Villemeur, Hervé |
| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
PLK4
MESH: Protein Transport MESH: Gene Expression Centriole MESH: Cyclin D3 Cell Biology/Cell Growth and Division lcsh:Medicine Gene Expression Mitosis [SDV.BC]Life Sciences [q-bio]/Cellular Biology Biology Protein Serine-Threonine Kinases MESH: Centrosome Cell Biology/Cell Signaling MESH: Protein-Serine-Threonine Kinases 03 medical and health sciences 0302 clinical medicine Microtubule Cell Biology/Cytoskeleton Gene duplication Humans MESH: Protein Binding Cyclin D3 lcsh:Science [SDV.BC] Life Sciences [q-bio]/Cellular Biology 030304 developmental biology Centrioles Centrosome 0303 health sciences Multidisciplinary MESH: Humans MESH: Centrioles lcsh:R MESH: Mitosis Cell biology MESH: Hela Cells Protein Transport 030220 oncology & carcinogenesis lcsh:Q Chromatid Cytokinesis HeLa Cells Protein Binding Research Article |
| Zdroj: | PLoS ONE PLoS ONE, 2011, 6 (1), pp.e14600. ⟨10.1371/journal.pone.0014600⟩ PLoS ONE, Public Library of Science, 2011, 6 (1), pp.e14600. ⟨10.1371/journal.pone.0014600⟩ PLoS ONE, Vol 6, Iss 1, p e14600 (2011) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0014600⟩ |
| Popis: | International audience; BACKGROUND: CDK11(p58) is a mitotic protein kinase, which has been shown to be required for different mitotic events such as centrosome maturation, chromatid cohesion and cytokinesis. METHODOLOGY/PRINCIPAL FINDINGS: In addition to these previously described roles, our study shows that CDK11(p58) inhibition induces a failure in the centriole duplication process in different human cell lines. We propose that this effect is mediated by the defective centrosomal recruitment of proteins at the onset of mitosis. Indeed, Plk4 protein kinase and the centrosomal protein Cep192, which are key components of the centriole duplication machinery, showed reduced levels at centrosomes of mitotic CDK11-depleted cells. CDK11(p58), which accumulates only in the vicinity of mitotic centrosomes, directly interacts with the centriole-associated protein kinase Plk4 that regulates centriole number in cells. In addition, we show that centriole from CDK11 defective cells are not able to be over duplicated following Plk4 overexpression. CONCLUSION/SIGNIFICANCE: We thus propose that CDK11 is required for centriole duplication by two non-mutually-exclusive mechanisms. On one hand, the observed duplication defect could be caused indirectly by a failure of the centrosome to fully maturate during mitosis. On the other hand, CDK11(p58) could also directly regulate key centriole components such as Plk4 during mitosis to trigger essential mitotic centriole modifications, required for centriole duplication during subsequent interphase. |
| Databáze: | OpenAIRE |
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