Toward Standardization of BK Virus Monitoring: Evaluation of the BK Virus R-gene Kit for Quantification of BK Viral Load in Urine, Whole-Blood, and Plasma Specimens
| Autor: | Morgane Solis, Pilar Domingo-Calap, Heidi Barth, Françoise Stoll-Keller, Samira Fafi-Kremer, Charlotte Sueur, Quentin Lepiller, Mariam Meddeb, Seiamak Bahram, Rachel Freitag, Sophie Caillard, Eric Soulier |
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| Rok vydání: | 2014 |
| Předmět: |
Microbiology (medical)
Urine Sciences du Vivant [q-bio]/Médecine humaine et pathologie Biology medicine.disease_cause Virology Genotype medicine Humans methods standards Whole blood Polyomavirus Infections Reproducibility of Results R gene Viral Load Molecular diagnostics virology BK virus Tumor Virus Infections Blood BK Virus Reagent Kits Diagnostic Viral load |
| Zdroj: | Journal of Clinical Microbiology. 52:4298-4304 |
| ISSN: | 1098-660X 0095-1137 |
| Popis: | Screening of BK virus (BKV) replication is recommended to identify patients at increased risk of BKV-associated diseases. However, the heterogeneity of molecular techniques hinders the establishment of universal guidelines for BKV monitoring. Here we aimed to compare the performance of the CE-marked BK virus R-gene kit (R-gene) to the performance of our in-house assay for quantification of BKV DNA loads (BKVL). A 12-specimen panel from the Quality Control for Molecular Diagnostics (QCMD) organization, 163 urine samples, and 88 paired specimens of plasma and whole blood (WB) from transplant recipients were tested. Both the R-gene and in-house assays showed a good correlation within the QCMD panel ( r = 0.995 and r = 0.989, respectively). BKVL were highly correlated between assays, although positive biases were observed with the in-house assay in analysis of urine (0.72 ± 0.83 log 10 copies/ml), plasma (1.17 ± 0.63 log 10 copies/ml), and WB (1.28 ± 0.37 log 10 copies/ml). Recalibration with a common calibrator significantly reduced the bias in comparisons between assays. In contrast, BKVL was underestimated with the in-house PCR in eight samples containing BKV genotype II, presenting point mutations at primer-annealing sites. Using the R-gene assay, plasma and WB specimens were found to be equally suitable for quantification of BKVL, as indicated by the high correlation coefficient ( r = 0.965, P < 0.0001). In conclusion, the R-gene assay demonstrated reliable performance and higher accuracy than the in-house assay for quantification of BKVL in urine and blood specimens. Screening of BKV replication by a well-validated commercial kit may enable clinical laboratories to assess viral loads with greater reproducibility and precision. |
| Databáze: | OpenAIRE |
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