Transporters involved in the hepatic uptake of (99m)Tc-mebrofenin and indocyanine green
| Autor: | Michal Heger, Bruno Stieger, Gert van Cappellen, Gerd A. Kullak-Ublick, Stephanie Häusler, Rolf Hesselmann, Thomas M. van Gulik, Tessa M van Ginhoven, Wilmar de Graaf, Roelof J. Bennink |
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| Přispěvatelé: | Surgery, Developmental Biology, University of Zurich, AGEM - Amsterdam Gastroenterology Endocrinology Metabolism, CCA -Cancer Center Amsterdam, AII - Amsterdam institute for Infection and Immunity, Nuclear Medicine |
| Rok vydání: | 2011 |
| Předmět: |
Organic anion transporter 1
genetic structures Organic Anion Transporters Pharmacology Organic Anion Transporters Sodium-Independent chemistry.chemical_compound Xenopus laevis 0302 clinical medicine Liver Function Tests Cricetinae 0303 health sciences Aniline Compounds Microscopy Confocal biology medicine.diagnostic_test Symporters Chemistry Liver-Specific Organic Anion Transporter 1 Chinese hamster ovary cell Organotechnetium Compounds Recombinant Proteins 3. Good health Organic anion-transporting polypeptide Liver 030220 oncology & carcinogenesis Female Indocyanine Green medicine.medical_specialty Bilirubin Glycine Organic Anion Transporters Sodium-Dependent 610 Medicine & health CHO Cells In Vitro Techniques Transfection 03 medical and health sciences Solute Carrier Organic Anion Transporter Family Member 1B3 Cricetulus Internal medicine medicine Animals Humans Radionuclide Imaging 030304 developmental biology Hepatology Imino Acids Transporter eye diseases Endocrinology 10199 Clinic for Clinical Pharmacology and Toxicology biology.protein Oocytes 2721 Hepatology Liver function Radiopharmaceuticals Liver function tests Indocyanine green |
| Zdroj: | Journal of hepatology Journal of Hepatology, 54(4), 738-745. Elsevier Journal of hepatology, 54(4), 738-745. Elsevier |
| ISSN: | 0168-8278 |
| Popis: | Background & Aims: (99m)Tc-mebrofenin hepatobiliary scintigraphy (HBS) and the indocyanine green (ICG) clearance test are used for the assessment of hepatic function before and after liver surgery. The hepatic uptake of (99m)Tc-mebrofenin and ICG is considered similar to the uptake of organic anions such as bilirubin and bile acids. Little is known about hepatic uptake mechanisms of both compounds and recent evidence suggests that the hepatic transporters for ICG and (99m)Tc-mebrofenin are distinct. The aim of this study was to identify the specific human hepatic transporters of (99m)Tc-mebrofenin and 1CG. Methods: The uptake of (99m)Tc-mebrofenin was investigated in cRNA-injected Xenopus laevis oocytes expressing human OATP1B1, OATP1B3, OATP2B1, or NTCP. Chinese hamster ovary (CHO) cells stably expressing OATP1B1, OATP1B3, OATP2B1, or NTCP were used as a mammalian expression system. ICG transport into CHO cells was additionally imaged with confocal microscopy. Results: We demonstrated that OATP1B1 and OATP1B3 are involved in the transport of (99m)Tc-mebrofenin. OATP1B1 showed an approximately 1.5-fold higher affinity for (99m)Tc-mebrofenin compared to OATP1B3. ICG is transported by OATP1B3 and NTCP. Conclusions: The transporter specificity of (99m)Tc-mebrofenin and ICG partially overlaps as both compounds are transported by OATP1B3. (99m)Tc-mebrofenin is also taken up by OATP1B1, whereas ICG is additionally transported by NTCP. (C) 2010 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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