Burkholderia phytofirmans sp. nov., a novel plant-associated bacterium with plant-beneficial properties

Autor: A. V. Sturz, Bernard R. Glick, J.D. van Elsas, Denis Faure, Birgit Reiter, Jerzy Nowak, Joana Falcão Salles, Angela Sessitsch, Tom Coenye, E. Ait Barka, G. Wang-Pruski, Peter Vandamme
Přispěvatelé: Institut des sciences du végétal (ISV), Centre National de la Recherche Scientifique (CNRS), Van Elsas lab
Rok vydání: 2005
Předmět:
Burkholderia phytofirmans
strain psjn
Plant Roots
in-vitro growth
4-Butyrolactone
RNA
Ribosomal
16S
Onions
cepacia complex
Carbon-Carbon Lyases
Phylogeny
Base Composition
0303 health sciences
Rhizosphere
growth-promoting rhizobacteria
biology
Fatty Acids
Nucleic Acid Hybridization
General Medicine
Bacterial Typing Techniques
RNA
Bacterial
Flagella
potato
DNA
Bacterial
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
food.ingredient
Burkholderia
endophytic colonization
Movement
Molecular Sequence Data
Rhizobacteria
DNA
Ribosomal
Microbiology
Burkholderia phenazinium
resistance
03 medical and health sciences
food
n-acylhomoserine lactones
Botany
enhancement
Ecology
Evolution
Behavior and Systematics
030304 developmental biology
Biointeracties and Plant Health
030306 microbiology
Genes
rRNA
Sequence Analysis
DNA
Paraburkholderia
biology.organism_classification
16S ribosomal RNA
identification
PRI Biointeractions en Plantgezondheid
Phenazines
Gentian Violet
Burkholderia fungorum
Zdroj: International Journal of Systematic and Evolutionary Microbiology
International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2005, 55, pp.1187-1192. ⟨10.1099/ijs.0.63149-0⟩
International Journal of Systematic and Evolutionary Microbiology 55 (2005) 3
International Journal of Systematic and Evolutionary Microbiology, 55, 1187-1192
International Journal of Systematic and Evolutionary Microbiology, 55(3), 1187-1192
ISSN: 1466-5034
1466-5026
Popis: A Gram-negative, non-sporulating, rod-shaped, motile bacterium, with a single polar flagellum, designated strain PsJNT, was isolated from surface-sterilized onion roots. This isolate proved to be a highly effective plant-beneficial bacterium, and was able to establish rhizosphere and endophytic populations associated with various plants. Seven related strains were recovered from Dutch soils. Based on 16S rRNA gene sequence data, strain PsJNT and the Dutch strains were identified as representing a member of the genus Burkholderia, as they were closely related to Burkholderia fungorum (98·7 %) and Burkholderia phenazinium (98·5 %). Analysis of whole-cell protein profiles and DNA–DNA hybridization experiments confirmed that all eight strains belonged to a single species. Strain PsJNT had a DNA G+C content of 61·0 mol%. Only low levels of DNA–DNA hybridization to closely related species were found. Qualitative and quantitative differences in fatty acid composition between strain PsJNT and closely related species were identified. The predominant fatty acids in strain PsJNT were 16 : 0, 18 : 1ω7c and summed feature 3 (comprising 16 : 1ω7c and/or iso-15 : 0 2-OH). Isolate PsJNT showed high 1-aminocyclopropane-1-carboxylate deaminase activity and is therefore able to lower the ethylene level in a developing or stressed plant. Production of the quorum-sensing signal compound 3-hydroxy-C8-homoserine lactone was detected. Based on the results of this polyphasic taxonomic study, strain PsJNT and the seven Dutch isolates are considered to represent a single, novel species, for which the name Burkholderia phytofirmans sp. nov. is proposed. The type strain is strain PsJNT (=LMG 22146T=CCUG 49060T).
Databáze: OpenAIRE
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