Total synthesis by modern chemical ligation methods and high resolution (1.1 Å) X-ray structure of ribonuclease A
| Autor: | Valentina Tereshko, David J. Boerema, Stephen B. H. Kent |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Protein Folding
Protein Conformation RNase P Molecular Sequence Data Biophysics Biochemistry Chemical synthesis Catalysis Protein Structure Secondary Biomaterials X-Ray Diffraction Hydrolase Animals Amino Acid Sequence Cysteine Disulfides Ribonuclease biology Chemistry Organic Chemistry Resolution (electron density) Water Total synthesis Hydrogen Bonding Ribonuclease Pancreatic General Medicine Native chemical ligation Combinatorial chemistry Kinetics Crystallography Models Chemical biology.protein Cattle Chemical ligation |
| Zdroj: | Biopolymers. 90:278-286 |
| ISSN: | 0006-3525 |
| DOI: | 10.1002/bip.20800 |
| Popis: | The total chemical synthesis of RNase A using modern chemical ligation methods is described, illustrating the significant advances that have been made in chemical protein synthesis since Gutte and Merrifield's pioneering preparation of RNase A in 1969. The identity of the synthetic product was confirmed through rigorous characterization, including the determination of the X-ray crystal structure to 1.1 Angstrom resolution. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text