Characterization of the picrotoxin site of GABAA receptors

Autor:	Ashok K. Mehta, Maharaj K. Ticku
Rok vydání:	2014
Předmět:	Pharmacology GABAA receptor Kinetics Allosteric regulation Cell Membrane Brain Bridged Bicyclo Compounds Heterocyclic Receptors GABA-A Binding Competitive In vitro GABAA-rho receptor Rats GABA Antagonists chemistry.chemical_compound Radioligand Assay nervous system chemistry Biochemistry Animals Picrotoxin Receptor IC50
Zdroj:	Current Protocols in Pharmacology
ISSN:	1934-8290
Popis:	This protocol describes an in vitro assay for characterization of the picrotoxin site of GABA(A) receptors in rat brain membranes using various radioligands. Methods and representative data for Scatchard analysis (Kd, Bmax determination), association kinetics, dissociation kinetics and competition assays (IC50, Ki determination) are included in the protocol.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::256ce0615d57c988ef20ce6432b87bfe https://pubmed.ncbi.nlm.nih.gov/24510752 Zobrazit plný text záznamu Plný text