Interaction of the human N-Ras protein with lipid raft model membranes of varying degrees of complexity
| Autor: | Jörg Nikolaus, Herbert Waldmann, Alexander Vogel, Katrin Weise, Roland Winter, Andreas Herrmann, Gemma Triola, Daniel Huster |
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| Rok vydání: | 2014 |
| Předmět: |
Sphingosine
Clinical Biochemistry Biological membrane Raft Models Biological Biochemistry Proto-Oncogene Proteins p21(ras) chemistry.chemical_compound Membrane Microdomains Membrane chemistry Fluorescence microscope Biophysics Humans lipids (amino acids peptides and proteins) Lipid bilayer phase behavior Molecular Biology POPC Lipid raft |
| Zdroj: | Biological Chemistry. 395:779-789 |
| ISSN: | 1437-4315 1431-6730 |
| DOI: | 10.1515/hsz-2013-0294 |
| Popis: | Ternary lipid mixtures composed of cholesterol, saturated (frequently with sphingosine backbone), and unsaturated phospholipids show stable phase separation and are often used as model systems of lipid rafts. Yet, their ability to reproduce raft properties and function is still debated. We investigated the properties and functional aspects of three lipid raft model systems of varying degrees of biological relevance – PSM/POPC/Chol, DPPC/POPC/Chol, and DPPC/DOPC/Chol – using 2H solid-state nuclear magnetic resonance (NMR) spectroscopy, fluorescence microscopy, and atomic force microscopy. While some minor differences were observed, the general behavior and properties of all three model mixtures were similar to previously investigated influenza envelope lipid membranes, which closely mimic the lipid composition of biological membranes. For the investigation of the functional aspects, we employed the human N-Ras protein, which is posttranslationally modified by two lipid modifications that anchor the protein to the membrane. It was previously shown that N-Ras preferentially resides in liquid-disordered domains and exhibits a time-dependent accumulation in the domain boundaries of influenza envelope lipid membranes. For all three model mixtures, we observed the same membrane partitioning behavior for N-Ras. Therefore, we conclude that even relatively simple models of raft membranes are able to reproduce many of their specific properties and functions. |
| Databáze: | OpenAIRE |
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