Telomere length and age-dependent telomere attrition: the blood-and-muscle model
| Autor: | Simon Toupance, Mirna N. Chahine, Carlos Labat, Sylvie Gautier, Toufic Moussallem, Pierre Yared, Roland Asmar, Athanase Benetos, Sandy H El-Hakim |
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| Přispěvatelé: | Neuroscience Research Center [Beirut, Lebanon] (Faculty of Medical Sciences), Lebanese University [Beirut] (LU), Défaillance Cardiovasculaire Aiguë et Chronique (DCAC), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL), Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy), Nancyclotep- Experimental Imaging Platform = Plate-forme d'imagerie moléculaire, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Université de Lorraine (UL), Faculty of Public Health [Fanar, Liban], Partially supported by a grant to Athanase Benetos from the French National Research Agency, Foundation-Medical Research Institute (F-MRI®) for partial financial support of this study, DE CARVALHO, Philippe |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Oncology
Male Physiology muscle [SDV]Life Sciences [q-bio] Age dependent Disease Body Mass Index Southern blot 0302 clinical medicine leucocytes Attrition Aged 80 and over 0303 health sciences [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology Atherosclerotic cardiovascular disease Smoking Diseases onset General Medicine vieillissement Middle Aged Telomere télomère [SDV.MHEP.CSC] Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system [SDV] Life Sciences [q-bio] medicine.anatomical_structure Female psychological phenomena and processes Blood drawing Adult medicine.medical_specialty leukocytes 03 medical and health sciences [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system Physiology (medical) Internal medicine buvardage de Southern medicine [SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular Biology Humans [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology Muscle Skeletal 030304 developmental biology Aged Pharmacology business.industry aging Skeletal muscle medicine.disease business 030217 neurology & neurosurgery [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Zdroj: | Canadian Journal of Physiology and Pharmacology Canadian Journal of Physiology and Pharmacology, NRC Research Press, 2019, 97 (4), pp.328-334. ⟨10.1139/cjpp-2018-0582⟩ |
| ISSN: | 0008-4212 1205-7541 |
| Popis: | International audience; Short telomere length (TL) is associated with atherosclerotic cardiovascular disease (ACVD) and other age-related diseases. It is unclear whether these associations originate from having inherently short TL or a faster TL attrition before or during disease development. We proposed the blood-and-muscle model to assess TL dynamics throughout life course. Our objective was to measure TL in leukocytes (LTL) and in skeletal muscle (MTL), which served as a proxy of TL at birth. The delta (MTL-LTL) represented life-long telomere attrition. Blood draws and skeletal muscle biopsies were performed on 35 Lebanese individuals undergoing surgery. Following DNA extraction, LTL and MTL were measured by Southern blot. In every individual aged between 30 and 85 years, MTL was longer than LTL. With age, MTL and LTL decreased, but the delta (MTL-LTL) increased by 14 bp/year. We validated the blood-and-muscle model that allowed us to identify TL, TL at birth, and lifelong TL attrition in a cross-sectional study. This model can be used in larger cross-sectional studies to evaluate the association of telomere dynamics with age-related diseases onset and progression. |
| Databáze: | OpenAIRE |
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