Use of Lysozyme as a Standard for Evaluating the Effectiveness of a Proteomics Process
| Autor: | Barry D. Hodge, Leah S. Riter, Randall K. Julian, Karen M. Gooding |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
Proteomics
Quality Control Analyte Chromatography Collision-induced dissociation Chemistry Blood Proteins General Chemistry Reference Standards Mass spectrometry Biochemistry High-performance liquid chromatography Molecular biology Mass Spectrometry Rats chemistry.chemical_compound Animals Process control Muramidase Sample preparation Lysozyme Chromatography High Pressure Liquid |
| Zdroj: | Journal of Proteome Research. 4:153-160 |
| ISSN: | 1535-3907 1535-3893 |
| DOI: | 10.1021/pr049819s |
| Popis: | Automated sequencing of unknowns in bottom-up proteomics makes the data produced susceptible to process control errors, which can be propagated into mistakes in analyte identification. Inclusion of an unintrusive internal standard, such as lysozyme, allows monitoring all phases of the proteomics process including sample preparation, enzymatic digestion, HPLC, mass spectrometry, and database searching. By using this internal standard, digestion issues including rearrangements, semi-tryptic fragments, and modifications were monitored. In addition, control of the HPLC process including column performance was achieved. The use of the lysozyme standard allowed easy optimization of mass spectral conditions including data dependent and collision induced dissociation settings. The use of this internal standard in a study of differential protein expression in rat serum samples is presented. |
| Databáze: | OpenAIRE |
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