Inhibition of migration and induction of apoptosis in LoVo human colon cancer cells by polysaccharides from Ganoderma lucidum
| Autor: | Qiu‑Long Hu, Zeng‑Enni Liang, You‑Jin Yi, Ren‑Cai Wang, Xing‑Yao Xiong, Yu‑Tong Guo |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Cancer Research
Reishi Colon Poly ADP ribose polymerase Cell Apoptosis Caspase 3 Biology Pharmacology Caspase 8 Biochemistry Cell Movement Polysaccharides Cell Line Tumor Genetics medicine Humans Cytotoxicity Molecular Biology Antineoplastic Agents Phytogenic Molecular biology Caspase 9 Enzyme Activation medicine.anatomical_structure Oncology Cell culture Colonic Neoplasms Molecular Medicine DNA fragmentation Poly(ADP-ribose) Polymerases Drugs Chinese Herbal |
| Zdroj: | Molecular Medicine Reports. 12:7629-7636 |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Ganoderma lucidum polysaccharides (GLPs), which were purified from the medicinal herb G. lucidum followed by ethanol precipitation, protein depletion using the Sevage assay, purification using DEAE‑cellulose (DE-52), dialysis and the use of ultrafiltration membranes, are used as an ingredient in traditional anticancer treatments in China. The aim of the current study was to evaluate the anticancer effects and investigate the underlying molecular mechanisms of GLPs on LoVo human colon cancer cells. The results demonstrated that the GLP‑mediated anticancer effect in LoVo cells was characterized by cytotoxicity, migration inhibition, enhanced DNA fragmentation, morphological alterations and increased lactate dehydrogenase release. Furthermore, the activation of caspases‑3, ‑8 and ‑9 was involved in GLP‑stimulated apoptosis. Additionally, treatment with GLPs promoted the expression of Fas and caspase‑3 proteins, whilst reducing the expression of cleaved poly(ADP‑ribose) polymerase. These data indicate that GLPs demonstrate potential antitumor activity in human colon cancer cells, predominantly through the inhibition of migration and induction of apoptosis. Furthermore, activation of the Fas/caspase-dependent apoptosis pathway is involved in the cytotoxicity of GLPs. |
| Databáze: | OpenAIRE |
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