Getting it right before transplantation: example of a stem cell model with regenerative potential for the CNS
Autor: | Cedric Viero, Govindan Dayanithi, Oksana Forostyak, Eva Syková |
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Přispěvatelé: | Saarland University [Saarbrücken], Czech Academy of Sciences [Prague] (CAS), Charles University [Prague] (CU), Mécanismes moléculaires dans les démences neurodégénératives (MMDN), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL) |
Jazyk: | angličtina |
Rok vydání: | 2014 |
Předmět: |
Pathology
medicine.medical_specialty Central nervous system Neuronal differentiation human embryonic stem cell calcium signaling 03 medical and health sciences Cell and Developmental Biology 0302 clinical medicine medicine neurodegenerative diseases Progenitor cell immortalized stem cell lines lcsh:QH301-705.5 030304 developmental biology 0303 health sciences neural precursors business.industry Calcium signalling Cell model spinal cord ion channels Cell Biology Embryonic stem cell Transplantation medicine.anatomical_structure lcsh:Biology (General) Perspective Article Stem cell line Stem cell business Neuroscience 030217 neurology & neurosurgery [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology Developmental Biology |
Zdroj: | Frontiers in Cell and Developmental Biology Frontiers in Cell and Developmental Biology, Frontiers media, 2014, 2, pp.36. ⟨10.3389/fcell.2014.00036⟩ Frontiers in Cell and Developmental Biology, Vol 2 (2014) |
ISSN: | 2296-634X |
DOI: | 10.3389/fcell.2014.00036 |
Popis: | International audience; The burden of neurodegenerative disorders in an aging population has become a challenge for the modern world. While the biomarkers available and the methods of diagnosis have improved to detect the onset of these diseases at early stages, the question of adapted and efficient therapies is still a major issue. The prospect of replacing the loss of functional neural cells remains an attractive but still audacious approach. A huge progress has been made in the generation of neurons derived from human stem cell lines and transplantation assays are tested in animals for a wide range of pathologies of the central nervous system. Here we take one step back and examine neuronal differentiation and the characterization of neural progenitors derived from human embryonic stem cells. We gather results from our previous studies and present a cell model that was successfully used in functional analyses and engraftment experiments. These neuronal precursors exhibit spontaneous and evoked activity, indicating that their electrophysiological and calcium handling properties are similar to those of matured neurons. Hence this summarized information will serve as a basis to design better stem cell-based therapies to improve neural regeneration. |
Databáze: | OpenAIRE |
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