Developmental changes in circulating IL-8/CXCL8 isoforms in neonates
| Autor: | Cheryl R. Killingsworth, Nikolai N. Voitenok, Robin K. Ohls, David A. Randolph, Akhil Maheshwari, Rakesh P. Patel, Svetlana Akalovich, Brian Sims, Michael B. Fallon, Sadiq S. Shaik |
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| Rok vydání: | 2008 |
| Předmět: |
Gene isoform
Adult medicine.medical_specialty Chemokine Neutrophils Swine Immunology Inflammation Enzyme-Linked Immunosorbent Assay Biochemistry Pentapeptide repeat Monocytes Article Internal medicine medicine Immunology and Allergy Animals Humans Protein Isoforms Interleukin 8 Molecular Biology Regulation of gene expression Fetus biology Interleukin-8 Infant Newborn Gene Expression Regulation Developmental Chemotaxis Hematology Molecular biology Endocrinology Animals Newborn biology.protein medicine.symptom Chemokines |
| Zdroj: | Cytokine. 46(1) |
| ISSN: | 1096-0023 |
| Popis: | Interleukin-8 (IL-8/CXCL8) is widely expressed in fetal tissues although inflammatory changes are not seen. Circulating IL-8 is comprised of an endothelial-derived [ala-IL-8](77) isoform and another, more potent [ser-IL-8](72) secreted by most other cells; [ala-IL-8](77) can be converted into [ser-IL-8](72) by proteolytic removal of an N-terminal pentapeptide from [ala-IL-8](77). In this study, we show [ala-IL-8](77) is the predominant circulating isoform of IL-8 in premature neonates but not in term neonates/adults, who have [ser-IL-8](72) as the major isoform. This isoform switch from the less potent [ala-IL-8](77) to [ser-IL-8](72) correlates with a maturational increase in the neutrophil chemotactic potency of plasma IL-8. The emergence of [ser-IL-8](72) as the major isoform is likely due to increased plasma [ala-IL-8](77)-convertase activity and/or changes in the cellular sources of IL-8. Developmental changes in IL-8 isoforms may serve to minimize its inflammatory effects in the fetus and also provide a mechanism to restore its full activity after birth. |
| Databáze: | OpenAIRE |
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