Effect of impurities on the matrix-assisted laser desorption mass spectra of single-stranded oligodeoxynucleotides
| Autor: | Juanita N. Wickham, Thomas A. Shaler, Kristin A. Sannes, Christopher H. Becker, Kuang Jen Wu |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
chemistry.chemical_classification
Base Sequence Sodium Inorganic chemistry Molecular Sequence Data Oligonucleotides Salt (chemistry) chemistry.chemical_element DNA Single-Stranded Ethylenediaminetetraacetic acid Mass spectrometry Chloride Analytical Chemistry chemistry.chemical_compound Matrix-assisted laser desorption/ionization chemistry Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization medicine Sodium dodecyl sulfate Sodium acetate medicine.drug |
| Zdroj: | Analytical chemistry. 68(3) |
| ISSN: | 0003-2700 |
| Popis: | The effect of impurities on the analysis of single-stranded DNA oligomers by the technique of matrix-assisted laser desorption/ionization with time-of-flight mass spectrometry has been studied using the matrix 3-hydroxypicolinic acid and 355-nm pulsed light. By mixing the DNA oligomers with different concentrations of impurities and recording mass spectra, limits are set on the tolerable level of a given impurity in a sample. The tolerance limits for sodium chloride, potassium chloride, sodium acetate, sodium fluoride, sodium dodecyl sulfate (SDS), and manganese(II) chloride were found to be approximately 10(-2) M. It was found that magnesium salts degraded the mass spectrum at much lower levels of 10(-4) M. The organic compounds tris(hydroxymethyl)aminomethane (Tris), urea, dithiothreitol (DTT), glycerol, and ethylenediaminetetraacetic acid (EDTA), when present as its ammonium salt, were tolerable at concentrations into the range of 0.25-0.5 M, while the organic polyamine compound spermine substantially degraded the mass spectrum at concentrations above 10(-2) M. When comparing these results for DNA analysis with previously reported limits for protein analysis, large differences are seen for some of the impurities tested. |
| Databáze: | OpenAIRE |
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