The effects of hyperthermia on the immunomodulatory properties of human umbilical cord vein mesenchymal stem cells (MSCs)
| Autor: | Mohamad Ali Rezaee, Mehdi Mohammadi, Mohammad Reza Rahmani, Ali Jalili, Shilan Hesami |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Hyperthermia Cancer Research Physiology Cellular differentiation Inflammation Lymphocyte proliferation Biology Umbilical cord Umbilical Cord Immunomodulation 03 medical and health sciences 0302 clinical medicine Immune system Physiology (medical) medicine Humans Cells Cultured Cell Proliferation Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Hyperthermia Induced medicine.disease Mixed lymphocyte reaction Coculture Techniques 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Immunology Leukocytes Mononuclear Cancer research Cytokines Lymphocyte Culture Test Mixed medicine.symptom |
| Zdroj: | International Journal of Hyperthermia. :1-8 |
| ISSN: | 1464-5157 0265-6736 |
| DOI: | 10.1080/02656736.2017.1309576 |
| Popis: | Hyperthermia can modulate inflammation and the immune response. Based on the recruitment of mesenchymal stem cells (MSCs) to inflamed tissues and the immunomodulatory properties of these cells, the aim of this study was to examine the effects of hyperthermia on the immunomodulatory properties of MSCs in a mixed lymphocyte reaction (MLR).Passages 4-6 of human umbilical cord vein mesenchymal stem cells were co-cultured in a two-way MLR. Cells in the hyperthermia groups were incubated at 41 °C for 45 min. A colorimetric assay was employed to examine the effects of MSCs on cell proliferation. The levels of IL-4 and TNF-α proteins in the cell culture supernatant were measured, and non-adherent cells were used for RNA extraction, which was then used for cDNA synthesis. RT-PCR was utilised to assess levels of IL-10, IL-17A, IL-4, TNF-α, TGF-β1, FOX PUCV-MSCs co-cultured in an MLR reduced lymphocyte proliferation at 37 °C, whereas hyperthermia attenuated this effect. Hyperthermia increased expression of IL-10, TGF-β1 and FOXP3 mRNAs in co-culture; however, no effects on IL-17A and IFN-γ were observed, and it reduced CXCL12 expression. In co-culture, IL-4 mRNA and protein increased at 37 °C, an effect that was reduced by hyperthermia. No considerable change in TNF-α mRNA expression was found in hyperthermia-treated cells.Hyperthermia increases cell proliferation of the peripheral blood mononuclear cells and modifies the cytokine profile in the presence of UCV-MSCs. |
| Databáze: | OpenAIRE |
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