Selective activation of murine V beta 8.2 bearing T cells by Pseudomonas exotoxin A
| Autor: | Michael L. Misfeldt, Roy D. LeGrand, Diane M. Dixon |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
CD4-Positive T-Lymphocytes
Virulence Factors T cell CD8 Antigens Receptors Antigen T-Cell alpha-beta T-Lymphocytes Immunology Bacterial Toxins Exotoxins Biology medicine.disease_cause Lymphocyte Activation Microbiology Mice Antigen medicine Pseudomonas exotoxin Animals Cells Cultured ADP Ribose Transferases T-cell receptor T lymphocyte Molecular biology Thymocyte medicine.anatomical_structure Pseudomonas aeruginosa Exotoxin CD8 |
| Zdroj: | Cellular immunology. 145(1) |
| ISSN: | 0008-8749 |
| Popis: | We have determined that Pseudomonas aeruginosa exotoxin A (PE) can selectively stimulate the proliferation of V beta bearing T lymphocytes. Murine thymocytes were fractionated by selective agglutination with peanut agglutinin (PNA) and the PNA- thymocytes, which represent mature thymocytes, were shown to be responsive to PE stimulation. In addition, mature peripheral T lymphocytes (nylon wool nonadherent splenocytes) were also observed to respond to PE stimulation. Both CD4+ and CD8+ splenic T lymphocyte populations proliferated in response to PE. Flow microfluorimetry analysis of PNA- thymocytes stimulated with PE indicated that V beta 8.2 bearing T cells were preferentially expanded. Thus, our data indicate that PE represents a microbial super antigen which stimulates murine thymocytes which bear the V beta 8.2 element of the T cell receptor. |
| Databáze: | OpenAIRE |
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