Molecular Identification of Pro-Excitogenic Receptor and Channel Phenotypes of the Deafferented Lumbar Motoneurons in the Early Phase after SCT in Rats
| Autor: | Benjun Ji, Bartosz Wojtaś, Małgorzata Skup |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Motor Neurons
Symporters Organic Chemistry General Medicine Receptors N-Methyl-D-Aspartate Catalysis Computer Science Applications Rats Inorganic Chemistry hyperexcitability spasticity spinal cord injury AMPAR NMDAR GlyR GABAAR KCC2 5-HTRs Phenotype Chlorides Spinal Cord Muscle Spasticity Quality of Life Animals Physical and Theoretical Chemistry Molecular Biology alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid Spectroscopy Spinal Cord Injuries gamma-Aminobutyric Acid |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 19; Pages: 11133 |
| ISSN: | 1422-0067 |
| Popis: | Spasticity impacts the quality of life of patients suffering spinal cord injury and impedes the recovery of locomotion. At the cellular level, spasticity is considered to be primarily caused by the hyperexcitability of spinal α-motoneurons (MNs) within the spinal stretch reflex circuit. Here, we hypothesized that after a complete spinal cord transection in rats, fast adaptive molecular responses of lumbar MNs develop in return for the loss of inputs. We assumed that early loss of glutamatergic afferents changes the expression of glutamatergic AMPA and NMDA receptor subunits, which may be the forerunners of the developing spasticity of hindlimb muscles. To better understand its molecular underpinnings, concomitant expression of GABA and Glycinergic receptors and serotoninergic and noradrenergic receptors, which regulate the persistent inward currents crucial for sustained discharges in MNs, were examined together with voltage-gated ion channels and cation-chloride cotransporters. Using quantitative real-time PCR, we showed in the tracer-identified MNs innervating extensor and flexor muscles of the ankle joint multiple increases in transcripts coding for AMPAR and 5-HTR subunits, along with a profound decrease in GABAAR, GlyR subunits, and KCC2. Our study demonstrated that both MNs groups similarly adapt to a more excitable state, which may increase the occurrence of extensor and flexor muscle spasms. |
| Databáze: | OpenAIRE |
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