Establishment and characterization of immortalized human cell lines from prostatic carcinoma and benign prostatic hyperplasia
| Autor: | P T Iype, L E Iype, M E Kaighn, M Verma |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Male
Cancer Research Oncogene Reverse Transcriptase Polymerase Chain Reaction Cell Cell Culture Techniques Prostatic Hyperplasia Prostatic Neoplasms Cancer DNA Neoplasm Hyperplasia Biology Cell cycle Genes p53 medicine.disease medicine.anatomical_structure Oncology Cell culture Karyotyping Cancer cell Tumor Cells Cultured Cancer research medicine Carcinoma Humans |
| Zdroj: | International Journal of Oncology. |
| ISSN: | 1791-2423 1019-6439 |
| DOI: | 10.3892/ijo.12.2.257 |
| Popis: | New human prostate cell lines were developed from prostatic carcinoma (BRF-41T) and BPH (BRF-55T). Primary cultures were initiated from cellular outgrowths of explanted tissues. A serum-free medium, BRFF-HPC1, was developed for growing human prostatic cancer cells. Cell strains were immortalized with pRSV-T plasmid to generate permanent cell lines that exhibited an epithelial morphology. Both cell lines expressed the epithelial cell markers, cytokeratins 8 and 18 as well as the prostatic marker, PSA, and the androgen receptor gene. They possess the H-ras, K-ras, and p53 genes. We hope that these new human prostatic cell lines will be useful as in vitro models for cancer research. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|