Differential expression of selectins by mouse brain capillary endothelial cells in vitro in response to distinct inflammatory stimuli
| Autor: | Roméo Cecchelli, Caroline Coisne, Yannick Delplace, Bénédicte Dehouck, Lucie Dehouck, Christelle Faveeuw, Florence Miller |
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| Přispěvatelé: | Laboratoire de Physiopathologie de la Barrière Hémato-Encéphalique (LBHE), Université d'Artois (UA), CELLIAL Technologies, This work was supported by grants from the Ministry of Research of France (fellowship to C. Coisne) and by the 'Conseil Régional du Nord-Pas-de-Calais' (fellowship to F. Miller)., We are very grateful to Pr B. Engelhardt (Theodor Kocher Institute, University of Bern, Switzerland) and Dr. J. Boucraut (Laboratoire NICN UMR 6184, Faculté de Médecine Timone, Marseille) to their generous gifts of Abs. The authors thank Dr. Valérie Buée for the critical reading of the manuscript. |
| Rok vydání: | 2005 |
| Předmět: |
Lipopolysaccharides
MESH: Cell Adhesion Molecules/metabolism medicine.medical_treatment MESH: Lipopolysaccharides/pharmacology MESH: Neuroglia/drug effects Fluorescent Antibody Technique MESH: Animals Newborn Mice 0302 clinical medicine MESH: Flow Cytometry/methods MESH: Animals MESH: Platelet Endothelial Cell Adhesion Molecule-1/metabolism Cells Cultured Cerebral Cortex 0303 health sciences biology MESH: Fluorescent Antibody Technique/methods Cell adhesion molecule MESH: Inflammation/chemically induced General Neuroscience MESH: Vascular Cell Adhesion Molecule-1/metabolism MESH: Selectins/metabolism Flow Cytometry Intercellular Adhesion Molecule-1 Cell biology MESH: Cytokines/pharmacology Endothelial stem cell Platelet Endothelial Cell Adhesion Molecule-1 Cytokine medicine.anatomical_structure Syngenic Cytokines medicine.symptom Neuroglia Selectin MESH: Cells Cultured MESH: Inflammation/metabolism MESH: Neuroglia/metabolism MESH: Cerebral Cortex/cytology MESH: Endothelial Cells/drug effects In vitro studies Vascular Cell Adhesion Molecule-1 Inflammation Blood–brain barrier MESH: Endothelial Cells/metabolism 03 medical and health sciences E- and P-selectin expression at the blood-brain barrier Antigens CD E-selectin medicine Animals [SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular Biology MESH: Mice 030304 developmental biology MESH: Antigens CD/metabolism Endothelial Cells MESH: Gene Expression Regulation/physiology MESH: Gene Expression Regulation/drug effects MESH: Intercellular Adhesion Molecule-1/metabolism Animals Newborn Gene Expression Regulation Immunology biology.protein Selectins Cell Adhesion Molecules 030217 neurology & neurosurgery |
| Zdroj: | Neuroscience Letters Neuroscience Letters, Elsevier, 2006, 392 (3), pp.216-220. ⟨10.1016/j.neulet.2005.09.028⟩ |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/j.neulet.2005.09.028⟩ |
| Popis: | International audience; Increased lymphocyte trafficking across blood-brain barrier (BBB) is a prominent and early event in inflammatory and immune-mediated CNS diseases. The adhesion molecules that control the entry of leukocytes into the brain have not been fully elucidated. Although the role of ICAM-1 and VCAM-1 has been well documented, the expression and role of selectins is still a matter of controversy. In a mouse syngenic in vitro BBB model, highly relevant for examining immunological events, mouse brain capillary endothelial cells (MBCECs) do not express selectins. Treatment of MBCECs with LPS, induced E- and P-selectin expression, whereas TNF-alpha or IFN-gamma treatments did not. Finally, P-selectin but not E-selectin expression was induced in IL-1beta treated MBCECs. Thus, our study suggests that diverse inflammatory stimuli could differentially regulate selectin expression at the BBB. |
| Databáze: | OpenAIRE |
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