In vitro evaluation of the potential therapeutic role of Dendropanax morbifera extract in ameliorating osteoporosis and resultant bone impairment using MC3T3-E1 cells
| Autor: | Sungeun Ahn, Muhammad Hanif Siddiqi, Deok-Chun Yang, Sera Kang, Hae Yong Noh, Dong Uk Yang |
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| Rok vydání: | 2017 |
| Předmět: |
Osteoporosis
Osteocalcin Core Binding Factor Alpha 1 Subunit Pharmacology 030226 pharmacology & pharmacy Bone morphogenetic protein 2 p38 Mitogen-Activated Protein Kinases 03 medical and health sciences Mice 0302 clinical medicine Osteogenesis medicine Animals Araliaceae Cell Proliferation Osteoblasts biology business.industry Plant Extracts Gene Expression Regulation Developmental Osteoblast Cell Biology General Medicine 3T3 Cells medicine.disease RUNX2 medicine.anatomical_structure 030220 oncology & carcinogenesis biology.protein Alkaline phosphatase Stem cell business Type I collagen Developmental Biology Signal Transduction |
| Zdroj: | In vitro cellulardevelopmental biology. Animal. 54(5) |
| ISSN: | 1543-706X |
| Popis: | Osteoporosis is a widespread musculoskeletal deformity that affects thousands of older people every year, leading to bone abnormalities and ultimately increasing the risk of bone fractures in both genders. It is considered a lethal disease causing death in thousands of people at the late stage of life. Dendropanax morbifera Leveille is a subtropical broad-leaved prevalent species in Korea. Extracts of the leaves, stems, roots, and seeds of D. morbifera have been used in traditional medicine for the treatment of numerous diseases such as diabetes, atherogenesis, skin disorders, and headaches. However, the anti-osteoporosis effects of D. morbifera have not been examined. The primary objectives of this study were to elucidate the anti-osteoporosis effect of D. morbifera extract through an in vitro study using pre-osteoblastic MC3T3-E1 cells. We found that D. morbifera strongly increased the expression of bone metabolic markers such as alkaline phosphatase (ALP) activity, type I collagen (Col-I) level, and mineralization. Additionally, D. morbifera extract also upregulated the mRNA expression levels of osteogenic genes including ALP, osteocalcin (OCN), osterix (Osx), and runt-related transcription factor 2 (Runx2) in MC3T3-E1 cells via upregulation of bone morphogenetic protein 2 (BMP-2)/p38 MAPK/JNK and Smad1/5/8 signaling pathways. Moreover, addition of D. morbifera significantly suppressed the inhibitory effect of SB203580 (p38 inhibitor). In conclusion, the current study demonstrated that D. morbifera extract significantly increased osteoblast differentiation and mineralization in MC3T3-E1 cells by regulating BMP-2/p38/JNK and Smad1/5/8. Our study might be helpful in the discovery and development of new anti-osteoporosis therapeutic agents. |
| Databáze: | OpenAIRE |
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