Emergence of the pandemic H1N1 2009 influenza A virus in swine herds in Poland
| Autor: | Iwona Markowska-Daniel, M. Porowski, Zygmunt Pejsak, Andrzej Kowalczyk, Kinga Urbaniak, Paweł Karbowiak, E. Kozak |
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| Rok vydání: | 2013 |
| Předmět: |
040301 veterinary sciences
Veterinary medicine Biology medicine.disease_cause pandemic h1n1 2009 H5N1 genetic structure Virus Microbiology 0403 veterinary science 03 medical and health sciences pcr SF600-1100 Pandemic Influenza A virus medicine Gene 030304 developmental biology 0303 health sciences swine influenza Phylogenetic tree Strain (biology) pigs Outbreak 04 agricultural and veterinary sciences Virology 3. Good health poland symptoms |
| Zdroj: | Bulletin of the Veterinary Institute in Pulawy, Vol 57, Iss 3, Pp 293-300 (2013) |
| ISSN: | 2300-3235 |
| DOI: | 10.2478/bvip-2013-0051 |
| Popis: | The outbreaks of pandemic H1N1 influenza A virus (pdm-like H1N1 2009), detected for the first time in farrow-to-finish farms in Poland, were described. The nasal swabs and lung tissue collected from diseased/dead animals were tested using molecular techniques (RRT-PCR, MRT-PCR, RT-PCR, SSG-PCR, sequencing) and virus isolation. The amplification of the genetic material extracted from the tested samples confirmed the presence of the M1 gene sequence of type A influenza virus. Using MRT-PCRs no products characteristic for HA and NA of any swine influenza virus subtypes were obtained. Using SSGPCR, products specific for pandemic HA and NA gene fragments were detected. Six new pdm-like H1N1 2009 strains were isolated and characterised. Phylogenetic analysis of the HA and NA genes revealed that they belong to one lineage with the pandemic strain A/California/04/2009 and other human strains, including human strains isolated in Poland in 2011. |
| Databáze: | OpenAIRE |
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