Hyperactive FOXO1 results in lack of tip stalk identity and deficient microvascular regeneration during kidney injury
| Autor: | Angela Huang, Chris Sparages, Roberto F. Nicosia, Jeremy S. Duffield, Lan T.H. Dang, Christopher S. Chen, Sarah Kate Read, Christine Yoon, Takahide Aburatani, Graham Marsh, Stella Alimperti, Suzanne Szak, Naoki Nakagawa, Ivan G. Gomez, Bryce G. Johnson, Shuyu Ren, Giovanni Ligresti, Alfred C. Aplin |
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| Rok vydání: | 2017 |
| Předmět: |
Adult
Male Vascular Endothelial Growth Factor A 0301 basic medicine Angiogenesis Biophysics Neovascularization Physiologic Bioengineering FOXO1 Kidney Article Biomaterials Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Humans PTEN Protein kinase B Cells Cultured PI3K/AKT/mTOR pathway biology Forkhead Box Protein O1 Regeneration (biology) Endothelial Cells Vascular Endothelial Growth Factor Receptor-2 Cell biology Mice Inbred C57BL Vascular endothelial growth factor 030104 developmental biology medicine.anatomical_structure chemistry Mechanics of Materials Microvessels Immunology Ceramics and Composites biology.protein 030217 neurology & neurosurgery |
| Zdroj: | Biomaterials. 141:314-329 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2017.07.010 |
| Popis: | Loss of the microvascular (MV) network results in tissue ischemia, loss of tissue function, and is a hallmark of chronic diseases. The incorporation of a functional vascular network with that of the host remains a challenge to utilizing engineered tissues in clinically relevant therapies. We showed that vascular-bed-specific endothelial cells (ECs) exhibit differing angiogenic capacities, with kidney microvascular endothelial cells (MVECs) being the most deficient, and sought to explore the underlying mechanism. Constitutive activation of the phosphatase PTEN in kidney MVECs resulted in impaired PI3K/AKT activity in response to vascular endothelial growth factor (VEGF). Suppression of PTEN in vivo resulted in microvascular regeneration, but was insufficient to improve tissue function. Promoter analysis of the differentially regulated genes in KMVECs suggests that the transcription factor FOXO1 is highly active and RNAseq analysis revealed that hyperactive FOXO1 inhibits VEGF-Notch-dependent tip-cell formation by direct and indirect inhibition of DLL4 expression in response to VEGF. Inhibition of FOXO1 enhanced angiogenesis in human bio-engineered capillaries, and resulted in microvascular regeneration and improved function in mouse models of injury-repair. |
| Databáze: | OpenAIRE |
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