Carnosic acid improves porcine early embryonic development by inhibiting the accumulation of reactive oxygen species
| Autor: | Wen-Jie Yu, Dan Luo, Yan-Xia Peng, Jia-Bao Zhang, Shuang Liang, Nam-Hyung Kim, Hao Jiang, Yue Xiao, Cheng-Zhen Chen, Sheng-Peng Li, Xuerui Yao, Bao Yuan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Homeobox protein NANOG
Porcine Swine Parthenogenesis Embryonic Development Apoptosis medicine.disease_cause Embryo development Embryo Culture Techniques chemistry.chemical_compound SOX2 Pregnancy medicine Animals Blastocyst Carnosic acid Cell Proliferation chemistry.chemical_classification Membrane Potential Mitochondrial Reactive oxygen species Gene Expression Profiling Glutathione Embryonic stem cell Cell biology Culture Media In Vitro Oocyte Maturation Techniques Oxidative Stress medicine.anatomical_structure chemistry Gene Expression Regulation embryonic structures Abietanes Pregnancy Animal Animal Science and Zoology Original Article Female Antioxidant Reactive Oxygen Species Oxidative stress |
| Zdroj: | The Journal of Reproduction and Development |
| ISSN: | 1348-4400 0916-8818 |
| Popis: | Carnosic acid (CA), a natural catechol rosin diterpene, is used as an additive in animal feeds and human foods. However, the effects of CA on mammalian reproductive processes, especially early embryonic development, are unclear. In this study, we added CA to parthenogenetically activated porcine embryos in an in vitro culture medium to explore the influence of CA on apoptosis, proliferation, blastocyst formation, reactive oxygen species (ROS) levels, glutathione (GSH) levels, mitochondrial membrane potential, and embryonic development-related gene expression. The results showed that supplementation with 10 μM CA during in vitro culture significantly improved the cleavage rates, blastocyst formation rates, hatching rates, and total numbers of cells of parthenogenetically activated porcine embryos compared with no supplementation. More importantly, supplementation with CA also improved GSH levels and mitochondrial membrane potential, reduced natural ROS levels in blastomeres, upregulated Nanog, Sox2, Gata4, Cox2, Itga5, and Rictor expression, and downregulated Birc5 and Caspase3 expression. These results suggest that CA can improve early porcine embryonic development by regulating oxidative stress. This study elucidates the effects of CA on early embryonic development and their potential mechanisms, and provides new applications for improving the quality of in vitro-developed embryos. |
| Databáze: | OpenAIRE |
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