Glucocorticoid receptors α and β are modulated sex specifically in human placentas of intrauterine growth restriction (IUGR)
| Autor: | Ulrich Andergassen, Viola Obermeier, Stefan Hutter, Paula Hepp, Maria Emilia Solano, Christina Kuhn, Sven Mahner, Doris Mayr, Petra C. Arck, Udo Jeschke, Julia Messner, Simone Hofmann |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male medicine.medical_specialty Placenta Intrauterine growth restriction Receptors Glucocorticoid Sex Factors Glucocorticoid receptor Western blot Downregulation and upregulation Pregnancy Internal medicine medicine Humans reproductive and urinary physiology Fetal Growth Retardation medicine.diagnostic_test business.industry Obstetrics and Gynecology General Medicine medicine.disease Endocrinology Real-time polymerase chain reaction medicine.anatomical_structure Nuclear receptor embryonic structures Immunohistochemistry Female business |
| Zdroj: | Archives of Gynecology and Obstetrics. 300:323-335 |
| ISSN: | 1432-0711 0932-0067 |
| Popis: | The objective of this study was to analyze the expression of the glucocorticoid receptor (GR) subtypes GRα and GRβ in placentas affected by intrauterine growth restriction (IUGR). We analyzed the sex-specific placental expression of GRα and GRβ in 23 IUGR and 40 control placentas using immunohistochemistry and immunofluorescence. The GR gene, also known as nuclear receptor subfamily 3 group C member 1 (NR3C1), mRNA production in trophoblast-like cell line BeWo after stimulation with prednisolone was analyzed using quantitative polymerase chain reaction (qPCR) and on the protein level using western blot analysis. GR subtypes showed a sex-specific upregulation in placentas from IUGR compared to control placentas. An increased expression of GRα was detectable in female placental tissue, whereas GRβ was increased in males. Our data support previous findings suggesting that the glucocorticoid metabolism plays a role in the pathophysiology of IUGR. Furthermore, the data suggest that the underlying molecular mechanisms differ between male and female cases. |
| Databáze: | OpenAIRE |
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