Reduced stress-associated FKBP5 DNA methylation together with gut microbiota dysbiosis is linked with the progression of obese PCOS patients
| Autor: | Miaoqiong Ou, Yongsong Chen, Fu Chen, Wencan Xu, Zhangran Chen, Xiaoping Yang, Luanhong Wang, Chujia Lin, Guoshu Yin, Guishan Chen, Huihuang Yin, Qingxia Huang, Hong Lin, Minjie Chen, Weichun Zhang, Lan Chen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
endocrine system diseases medicine.medical_treatment Gut flora Applied Microbiology and Biotechnology Microbial ecology chemistry.chemical_compound 0302 clinical medicine biology QR100-130 Biodiversity Middle Aged Polycystic ovary female genital diseases and pregnancy complications DNA methylation Metabolome Female Disease Susceptibility Biotechnology Polycystic Ovary Syndrome Adult medicine.medical_specialty 030209 endocrinology & metabolism Microbiology Article Anovulation Tacrolimus Binding Proteins 03 medical and health sciences Young Adult Dehydroepiandrosterone sulfate Estrone sulfate Stress Physiological Internal medicine medicine Humans Metabolomics Obesity Clinical microbiology business.industry Insulin Computational Biology nutritional and metabolic diseases DNA Methylation medicine.disease biology.organism_classification Gastrointestinal Microbiome 030104 developmental biology Endocrinology chemistry Case-Control Studies Dysbiosis business Biomarkers |
| Zdroj: | npj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-12 (2021) NPJ Biofilms and Microbiomes |
| ISSN: | 2055-5008 |
| Popis: | Polycystic ovary syndrome (PCOS) is a common endocrine disease in females that is characterized by hyperandrogenemia, chronic anovulation, and polycystic ovaries. However, the exact etiology and pathogenesis of PCOS are still unknown. The aim of this study was to clarify the bacterial, stress status, and metabolic differences in the gut microbiomes of healthy individuals and patients with high body mass index (BMI) PCOS (PCOS-HB) and normal BMI PCOS (PCOS-LB), respectively. Here, we compared the gut microbiota characteristics of PCOS-HB, PCOS-LB, and healthy controls by 16S rRNA gene sequencing, FK506-binding protein 5 (FKBP5) DNA methylation and plasma metabolite determination. Clinical parameter comparisons indicated that PCOS patients had higher concentrations of total testosterone, androstenedione, dehydroepiandrosterone sulfate, luteinizing hormone, and HOMA-IR while lower FKBP5 DNA methylation. Significant differences in bacterial diversity and community were observed between the PCOS and healthy groups but not between the PCOS-HB and PCOS-LB groups. Bacterial species number was negatively correlated with insulin concentrations (both under fasting status and 120 min after glucose load) and HOMA-IR but positively related to FKBP5 DNA methylation. Compared to the healthy group, both PCOS groups had significant changes in bacterial genera, including Prevotella_9, Dorea, Maihella, and Slackia, and plasma metabolites, including estrone sulfate, lysophosphatidyl choline 18:2, and phosphatidylcholine (22:6e/19:1). The correlation network revealed the complicated interaction of the clinical index, bacterial genus, stress indices, and metabolites. Our work links the stress responses and gut microbiota characteristics of PCOS disease, which might afford perspectives to understand the progression of PCOS. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|