Retinoic acid treated HL60 cells express CEACAM1 (CD66a) and phagocytoseNeisseria gonorrhoeae
| Autor: | Pei Zhang, James Parker, Milica Pantelic, Inês Chen, Tie Chen, Fritz Grunert |
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| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
Neutrophils Phagocytosis Cellular differentiation Immunology Retinoic acid HL-60 Cells Tretinoin medicine.disease_cause Microbiology chemistry.chemical_compound Antigen Antigens CD Escherichia coli medicine Humans Immunology and Allergy Dimethyl Sulfoxide Antigens Bacterial biology Cell Differentiation General Medicine Antigens Differentiation Neisseria gonorrhoeae Infectious Diseases chemistry biology.protein Antibody Cell Adhesion Molecules medicine.drug |
| Zdroj: | FEMS Immunology & Medical Microbiology. 42:261-266 |
| ISSN: | 1574-695X 0928-8244 |
| Popis: | Neisseria gonorrhoeae (gonococci, GC) are phagocytosed by neutrophils through the interaction between opacity proteins (Opa) and the CEA (CD66) family of antigens. In order to study this interaction, we used the human myeloid leukemia HL60 cell line, which differentiates into granulocyte-like cells upon treatment with dimethylsulfoxide (DMSO) or retinoic acid (RA). We found that RA-, but not DMSO- or untreated-HL60 cells, can phagocytose OpaI-expressing gonococci as well as Escherichia coli. The interaction of OpaI E. coli with RA-treated HL60 cells was inhibited by antibodies against CEACAM1. Phagocytosis of OpaI E. coli was found to be a result of the expression of CEACAM1 in RA-treated HL60 cells. Our results indicate that the level of expression of CEACAM1 in HL60 cells can be regulated by treatment with RA in a differentiation-dependent manner, and that this is important for phagocytosis of OpaI-expressing gonococci or E. coli. |
| Databáze: | OpenAIRE |
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