Osteogenic response of mesenchymal stem cells to continuous mechanical strain is dependent on ERK1/2-Runx2 signaling
| Autor: | Lingyong Jiang, Bing Fang, Zonglai Jiang, Peng Zhang, Yuqiong Wu |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
MAP Kinase Signaling System
Cellular differentiation Bone Marrow Cells Core Binding Factor Alpha 1 Subunit Models Biological Bone remodeling Rats Sprague-Dawley stomatognathic system Osteogenesis Nitriles Butadienes Genetics medicine Animals Mechanotransduction Bone regeneration Cell Proliferation Osteoblasts Staining and Labeling Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells Osteoblast General Medicine Alkaline Phosphatase Rats Cell biology RUNX2 medicine.anatomical_structure Gene Expression Regulation Immunology Stress Mechanical Signal transduction |
| Zdroj: | International Journal of Molecular Medicine. |
| ISSN: | 1791-244X 1107-3756 |
| DOI: | 10.3892/ijmm.2012.934 |
| Popis: | Mechanical stimuli are responsible for bone remodeling during orthodontic tooth movement. The role of mechanical stimulation in the regulation of the fate of bone mesenchymal stem cells (BMSCs) is of interest in bone regeneration and tissue engineering applications. However, the signaling pathway involved in strain-induced biochemical events in BMSCs is not well established and can be controversial. This study investigated strain-induced proliferation and differentiation of BMSCs, as well as the mechanism of mechanotransduction. BMSCs were exposed to continuous mechanical strain (CMS) of 10% at 1 Hz. The results showed that CMS reduced the proliferation of BMSCs and stimulated osteogenic differentiation by activating Runx2, followed by increased alkaline phosphatase (ALP) activity and mRNA expression of osteogenesis-related genes (ALP, collagen type I and osteocalcin). Furthermore, the phosphorylation level of extracellular regulated protein kinase (ERK)1/2 increased significantly at the onset of strain. However, the presence of U0126, a selective inhibitor of ERK1/2, blocked the induction of Runx2 and subsequent osteogenic events. These findings demonstrate that CMS regulated Runx2 activation and favored osteoblast differentiation through activation of the ERK1/2 signaling pathway. These results will contribute to a better understanding of strain-induced bone remodeling and will form the basis for the correct choice of applied force in orthodontic treatment. |
| Databáze: | OpenAIRE |
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