Involvement of CRH in fever induced by a distinct pre-formed pyrogenic factor (PFPF)
| Autor: | G E P Souza, A. R. Zampronio, M. C. C. Melo, S.J. Hopkins |
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| Rok vydání: | 2000 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Necrosis Fever Corticotropin-Releasing Hormone Immunology Indomethacin Prostaglandin Pronase chemistry.chemical_compound In vivo Internal medicine medicine Animals Rats Wistar Chemokine CCL4 Dexamethasone Pharmacology business.industry Pyrogens Tumor Necrosis Factor-alpha Interleukin-8 Antagonist Interleukin Macrophage Inflammatory Proteins Rats Endocrinology chemistry Hypothalamus medicine.symptom business hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Inflammation research : official journal of the European Histamine Research Society ... [et al.]. 49(9) |
| ISSN: | 1023-3830 |
| Popis: | Objective: This study investigated the role of corticotrophin-releasing hormone (CRH) in mediating the fever induced by a novel pre-formed pyrogenic factor (PFPF), using a CRF antagonist in vivo and evaluating the capacity of PFPF to stimulate CRH release from the hypothalamus in vitro.¶Materials and Methods: Male Wistar rats were used. The PFPF, induced following brief incubation of rat peritoneal macrophages with LPS and retained on 10 or 20 kDa MW cut-off membranes, was injected intracerebroventricularly. Fever was monitored using a rectal probe. Hypothalamus tissue was incubated with PFPF to establish its ability to induce CRH release. The CRH was measured by ELISA.¶Results: PFPF induced a dose-dependent fever that was abolished by boiling or pronase treatment. Whereas both dexamethasone and indomethacin were effective in reducing interleukin- (IL) 1β-induced fever, only dexamethasone abolished the fever induced by PFPF. The CRH antagonist, α-helical CRH9-41, abolished the fever induced by synthetic CRH, IL-8 and PFPF but not tumour necrosis factor-α (TNF-α). Like IL-1, PFPF was able to induce the release of CRH from rat hypothalamic tissue in vitro.¶Conclusions: These results suggest that the fever induced by PFPF depends on CRH release but not prostaglandin synthesis.¶ |
| Databáze: | OpenAIRE |
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