Methyl Jasmonate Affects Morphology, Number and Activity of Endoplasmic Reticulum Bodies in Raphanus sativus Root Cells

Autor: Maxime Gotté, Sophie Bernard, Azeddine Driouich, Eric Nguema-Ona, Ikuko Hara-Nishimura, Rajgourab Ghosh, Maïté Vicré-Gibouin
Přispěvatelé: Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale (Glyco-MEV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie (PRIMACEN), Normandie Université (NU)-Normandie Université (NU)-Institute for Research and Innovation in Biomedicine (IRIB), Normandie Université (NU)-Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), Kyoto University [Kyoto], Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Rok vydání: 2014
Předmět:
0106 biological sciences
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Physiology
Raphanus
[SDV.BC.BC]Life Sciences [q-bio]/Cellular Biology/Subcellular Processes [q-bio.SC]
Plant Science
Acetates
Endoplasmic Reticulum
Methyl jasmonate
Plant Roots
01 natural sciences
chemistry.chemical_compound
Plant Growth Regulators
Genes
Reporter
[SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]
Plant defense against herbivory
Arabidopsis thaliana
ComputingMilieux_MISCELLANEOUS
[SDV.BDD.GAM]Life Sciences [q-bio]/Development Biology/Gametogenesis
Plant Proteins
Microscopy
0303 health sciences
biology
Chemistry
Raphanus sativus
Plant physiology
[SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular Networks [q-bio.MN]
General Medicine
Plants
Genetically Modified
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biomolecules [q-bio.BM]
Cell biology
Cyclopentanes
03 medical and health sciences
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Genomics [q-bio.GN]
Botany
Oxylipins
[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Biochemistry [q-bio.BM]
Root cap
030304 developmental biology
Endoplasmic reticulum
PYK10
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
Cell Biology
biology.organism_classification
Enzyme assay
[SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy
[SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding
[CHIM.POLY]Chemical Sciences/Polymers
Root
Seedlings
biology.protein
ER bodies
010606 plant biology & botany
Zdroj: Plant and Cell Physiology
Plant and Cell Physiology, Oxford University Press (OUP), 2015, 56 (1), pp.61-72. ⟨10.1093/pcp/pcu141⟩
ISSN: 1471-9053
0032-0781
Popis: The endoplasmic reticulum (ER) bodies are ER-derived structures that are found in Brassicaceae species and thought to play a role in defense. Here, we have investigated the occurrence, distribution and function of ER bodies in root cells of Raphanus sativus using a combination of microscopic and biochemical methods. We have also assessed the response of ER bodies to methyl jasmonate (MeJA), a phytohormone that mediates plant defense against wounding and pathogens. Our results show that (i) ER bodies do occur in different root cell types from the root cap region to the differentiation zone; (ii) they do accumulate a PYK10-like protein similar to the major marker protein of ER bodies that is involved in defense in Arabidopsis thaliana; and (iii) treatment of root cells with MeJA causes a significant increase in the number of ER bodies and the activity of β-glucosidases. More importantly, MeJA was found to induce the formation of very long ER bodies that results from the fusion of small ones, a phenomenon that has not been reported in any other study so far. These findings demonstrate that MeJA impacts the number and morphology of functional ER bodies and stimulates ER body enzyme activities, probably to participate in defense responses of radish root. They also suggest that these structures may provide a defensive system specific to root cells.
Databáze: OpenAIRE
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