Oral dosing of rodents using a palatable tablet
| Autor: | Christoffer Bundgaard, David S. Tait, Shuang Xia, Sandeep S. Dhawan, Verity J. Brown, Ellen E. Bowman |
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| Přispěvatelé: | University of St Andrews. School of Psychology and Neuroscience, University of St Andrews. Institute of Behavioural and Neural Sciences |
| Rok vydání: | 2018 |
| Předmět: |
Male
Locomotor activity 0301 basic medicine BF Psychology media_common.quotation_subject medicine.medical_treatment Intraperitoneal injection NDAS Administration Oral BF Rodentia Modafinil R Medicine (General) Pharmacology 03 medical and health sciences Route of administration 0302 clinical medicine SDG 3 - Good Health and Well-being Pharmacokinetics Oral administration Animals Medicine Oral Administration Original Investigation media_common Gastrointestinal tract business.industry Appetite R1 Rats Bioavailability 030104 developmental biology Taste Gelatin Central Nervous System Stimulants Female business Locomotion 030217 neurology & neurosurgery Tablets medicine.drug |
| Zdroj: | Psychopharmacology |
| ISSN: | 1432-2072 0033-3158 |
| DOI: | 10.1007/s00213-018-4863-2 |
| Popis: | Rationale: Delivering orally bioavailable drugs to rodents is an important component to investigating that route of administration in novel treatments for humans. However, the traditional method of oral gavage requires training, is stressful, and can induce oesophageal damage in rodents. Objectives: To demonstrate a novel administrative technique – palatable gelatine tablets – as a stress-free route of oral delivery. Methods: 24 male Lister hooded rats were sacrificed for brain tissue analysis at varying time-points after jelly administration of 30 mg/kg of the wake-promoting drug modafinil. A second group of 22 female rats were tested on locomotor activity after 30 mg/kg modafinil, or after vehicle jellies, with the locomotor data compared to the brain tissue concentrations at the corresponding times. Results: Modafinil was present in the brain tissue at all time-points, reducing in concentration over time. The pattern of brain tissue modafinil concentration is comparable to previously reported results following oral gavage. Modafinil-treated rats were more active than control rats, with greater activity during the later time-periods – similar to that previously reported following intraperitoneal injection of 40 mg/kg modafinil. Conclusions: Palatable jelly tablets are an effective route of administration of thermally-stable orally-bioavailable compounds, eliminating the stress/discomfort and health risk of oral gavage and presenting as an alternative to previously reported palatable routes of administration where high protein and fat levels may adversely affect appetite for food reward, and uptake rate in the gastrointestinal tract. Publisher PDF |
| Databáze: | OpenAIRE |
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