Preliminary observations of genetic susceptibility of elk (Cervus elaphus nelsoni) to chronic wasting disease by experimental oral inoculation
| Autor: | Amir N. Hamir, Thomas Cline, Michelle Crocheck, Katherine I. O'Rourke, Thomas Gidlewski, Terry R. Spraker, Lynn H. Creekmore, Janice M. Miller |
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| Rok vydání: | 2006 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Genotype 040301 veterinary sciences Lymphoid Tissue Prions animal diseases Blotting Western Biology 0403 veterinary science Cervus elaphus nelsoni 03 medical and health sciences fluids and secretions Genetic predisposition medicine Animals Genetic Predisposition to Disease Prion protein Allele Alleles DNA Primers Brain Chemistry General Veterinary Inoculation Deer Brain 04 agricultural and veterinary sciences Chronic wasting disease medicine.disease Virology Immunohistochemistry 030104 developmental biology Prnp gene Wasting Disease Chronic |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 18(1) |
| ISSN: | 1040-6387 |
| Popis: | To compare the genetic susceptibility of elk ( Cervus elaphus nelsoni) with various alleles of the PRNP gene, which encodes the normal cellular prion protein, to chronic wasting disease (CWD), eight 8-month-old elk calves of 3 genotypes (2 132MM, 2 132LM, and 4 132LL) were orally dosed with CWD-infected brain material from elk. During postinoculation (PI) month 23, both 132MM elk had lost appetite, developed clinical signs of weight loss and central nervous system (CNS) dysfunction, and were euthanized. Two other elk (both 132LM) developed similar clinical signs of disease and were euthanized during PI month 40. All 4 affected elk had microscopic lesions of spongiform encephalopathy (SE), and PrPres, the disease-associated form of the prion protein, was detected in their CNS and lymphoid tissues by use of immunohistochemical (IHC) and Western blot (WB) techniques. These findings indicate that elk with MM and LM at codon 132 are susceptible to orally inoculated CWD. All 4 LL elk are alive at PI year 4 and are clinically normal, which suggests that 132LL elk may have reduced susceptibility to oral infection with CWD-infected material or may have prolonged incubation time. |
| Databáze: | OpenAIRE |
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