Human umbilical cord mesenchymal stem cells-derived extracellular vesicles facilitate the repair of spinal cord injury via the miR-29b-3p/PTEN/Akt/mTOR axis
Autor: | Zhang Zhen, Zhang Yilu, Li Weiwei, Xu Zhenchao, Xiao Xiao, Ye Hongru, Rong Dingchao, Wu Yunqi, Xiyang Wang, Xie Liqiong |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Cancer Research
Immunology Umbilical cord Article Cellular and Molecular Neuroscience medicine PTEN Spinal cord injury Protein kinase B PI3K/AKT/mTOR pathway RC254-282 biology QH573-671 business.industry Mesenchymal stem cell Neoplasms. Tumors. Oncology. Including cancer and carcinogens Cell Biology medicine.disease Spinal cord Cellular neuroscience Stem-cell research medicine.anatomical_structure Cancer research biology.protein NeuN business Cytology |
Zdroj: | Cell Death Discovery, Vol 7, Iss 1, Pp 1-10 (2021) Cell Death Discovery |
ISSN: | 2058-7716 |
Popis: | Spinal cord injury (SCI) is a salient traumatic disease that often leads to permanent disability, and motor and sensory impairments. Human umbilical cord mesenchymal stem cells (HucMSCs) have a wide application prospect in the treatment of SCI. This study explored the repair effect of HucMSCs-derived extracellular vesicles (HucMSCs-EVs) on SCI. HucMSCs and HucMSCs-EVs were cultured and identified. The rat model of SCI was established, and SCI rats were treated with HucMSCs-EVs. The motor function of SCI rats and morphology of spinal cord tissues were evaluated. Levels of NeuN, GFAP, and NF200 in spinal cord tissues were detected and cell apoptosis was measured. SCI rats were treated with EVs extracted from miR-29b-3p inhibitor-transfected HucMSCs. The downstream gene and pathway of miR-29b-3p were examined. HucMSCs-EVs-treated rats showed obvious motor function recovery and reduced necrosis, nuclear pyknosis, and cavity. HucMSCs-EVs alleviated spinal cord neuronal injury. miR-29b-3p was poorly expressed in SCI tissues, but highly expressed in EVs and SCI rats treated with EVs. miR-29b-3p targeted PTEN. Inhibition of miR-29b-3p or overexpression of PTEN reversed the repair effect of EVs on SCI. EVs activated the AKT/mTOR pathway via the miR-29b-3p/PTEN. In conclusion, HucMSCs-EVs reduced pathological changes, improved motor function, and promoted nerve function repair in SCI rats via the miR-29b-3p/PTEN/Akt/mTOR axis. |
Databáze: | OpenAIRE |
Externí odkaz: |