Adipose-derived mesenchymal stem cells preserve cardiac function via ANT-1 in dilated cardiomyopathy hamster model
| Autor: | Yoshiki Sawa, Kotoe Kawai, Toru Kuratani, Miwa Oota, Hiroki Hata, Takashi Kido, Takayoshi Ueno, Shigeru Miyagawa, Hiroyuki Nishida, Hayato Kurata, Daisuke Mori, Koichi Toda |
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| Rok vydání: | 2021 |
| Předmět: |
Medicine (General)
medicine.medical_specialty VEGF Vascular endothelial growth factor Biomedical Engineering Hamster Adipose tissue ADSC Adipose tissue-derived mesenchymal stem cells LV Left ventricle Biomaterials R5-920 Fibrosis Internal medicine Myosin Idiopathic dilated cardiomyopathy medicine QH573-671 Chemistry Mesenchymal stem cell Dilated cardiomyopathy ANOVA Analyses of variance medicine.disease ATP Transplantation SEM Standard error of the mean Endocrinology TGFR Transforming growth factor receptor EF Ejection fraction Mitochondrial transporter HGF Human growth factor Mesenchymal stem cells Original Article Cytology OD Optical density Developmental Biology |
| Zdroj: | Regenerative Therapy, Vol 18, Iss, Pp 182-190 (2021) Regenerative Therapy |
| ISSN: | 2352-3204 |
| Popis: | Introduction Idiopathic dilated cardiomyopathy (DCM) is associated with abnormalities in cytoskeletal proteins, mitochondrial ATP transporter, microvasculature, and fibrosis. Mesenchymal stem cells (MSCs) can ameliorate distressed mitochondrial and structural proteins, as well as fibrosis, via the paracrine effect of cytokines. This study aimed to investigate whether the transplantation of adipose tissue-derived MSCs (ADSCs) reverses histological and functional abnormalities in the distressed myocardium of DCM-like hamsters by modulating the expression of adenine nucleotide translocase 1 (ANT-1). Methods Eighteen weeks after birth, ADSCs were implanted onto the cardiac surface of δ-sarcoglycan (SG)-deficient hamsters or sham surgery was performed. Results Left ventricular ejection fraction and end-systolic diameter were maintained in ADSC-treated animals for four weeks, ATP concentration was considerably elevated in the cardiomyocytes of these animals, and ANT-1 expression was significantly upregulated as well. The expression of extracellular matrix and myocardial cytoskeletal proteins, such as collagen, SG, and α-dystroglycan, did not differ between groups. However, significant improvements in myosin and Smad4 expression, cardiomyocyte hypertrophy, and capillary density occurred in the ADSC-treated group. Conclusions We demonstrated that ADSCs might maintain cardiac function in the DCM hamster model by enhancing ATP concentration, as well as mitochondrial transporter and myosin expression, indicating their potential for DCM treatment. |
| Databáze: | OpenAIRE |
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